食品科学 ›› 2010, Vol. 31 ›› Issue (11): 31-37.doi: 10.7506/spkx1002-6630-201011007

• 基础研究 • 上一篇    下一篇

石榴皮多酚纯化及其抗氧化活性表征

赵艳红,李建科*,李国荣   

  1. 陕西师范大学食品工程与营养科学学院
  • 收稿日期:2009-10-11 出版日期:2010-06-01 发布日期:2010-12-29
  • 通讯作者: 李建科 E-mail:jiankel@snnu.edu.cn
  • 基金资助:

    国家大学生创新性实验计划项目(081071813);陕西师范大学研究生培养创新基金项目(2009CXS022);西安市科技计划项目(NC09056)

Purification with Macroporous Adsorbent Resins and in vitro Antioxidant Evaluation of Pomegranate Peel Polyphenols

ZHAO Yan-hong LI Jian-ke* LI Guo-rong   

  1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi an 710062, China
  • Received:2009-10-11 Online:2010-06-01 Published:2010-12-29
  • Contact: LI Jian-ke E-mail:jiankel@snnu.edu.cn

摘要:

目的:研究石榴皮多酚的纯化技术以及对石榴皮多酚提取物进行体外抗氧化活性表征。方法:选用国产10种大孔吸附树脂对超声波辅助提取所得的石榴皮粗提物进行分离纯化,并研究吸附动力学特征。采用4种常用体外抗氧化评价方法对纯化的石榴皮多酚进行活性表征。结果:大孔吸附树脂D160和XRD-6使石榴皮多酚粗提物中多酚纯度由35.05%提高到87.81%,其中,安石榴苷的纯度由原来的21.25%提高到73.96%。体外抗氧化实验表明,抗氧化活性与石榴皮提取物中多酚的纯度,尤其是安石榴苷的纯度,呈明显的剂量-效应关系;结论:大孔吸附树脂可以应用于石榴皮多酚的分离纯化;安石榴苷是石榴皮多酚中起抗氧化活性的关键成分。

关键词: 石榴皮多酚, 大孔吸附树脂, 纯化, 抗氧化

Abstract:

Objective: To purify pomegranate peel polyphenols with macroporous adsorbent resins and evaluate their antioxidant activity in vitro. Methods: The crude polyphenol extract obtained from pomegranate peel through ultrasound-assisted extraction was separated and purified with 10 kinds of domestic macroporous adsorbent resins, and the adsorption kinetics was investigated. In addition, four classical antioxidant assessment methods were used to evaluate the antioxidant activity of the purified pomegranate peel polyphenols. Results: The content of pomegranate peel polyphenols in the crude extract was increased from 35.05% to 87.81% using macroporous adsorbent resins D160 and XRD-6 and the content of punicalagin was increased from 21.25% to 73.96%. in vitro antioxidant activity of the extract exhibited a dose-effect relationship relevant to the content of polyphenols, especially punicalagin. Conclusion: The application of macroporous adsorbent resins appears to be feasible for the separation and purification of pomegranate peel extract, and punicalagin is a key antioxidant component in pomegranate peel polyphenol mixtures.

Key words: pomegranate peel polyphenol, macroporous adsorbent resins, purification, antioxidation

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