食品科学 ›› 2010, Vol. 31 ›› Issue (11): 162-167.doi: 10.7506/spkx1002-6630-201011035

• 生物工程 • 上一篇    下一篇

屎肠球菌BC-3 产类细菌素发酵培养基的优化

闵钟熳,岳喜庆* ,蔡玮璠,郭 晨   

  1. 沈阳农业大学食品学院
  • 收稿日期:2009-11-05 修回日期:2010-02-06 出版日期:2010-06-01 发布日期:2010-12-29
  • 通讯作者: 岳喜庆 E-mail:minzhongman@gmail.com
  • 基金资助:

    辽宁省创新团队项目(2009T085)

Optimization of Fermentation Culture Medium for Enterococcus faecium BC-3 for the Production of Bacteriocin-like Substances

MIN Zhong-man,YUE Xi-qing*,CAI Wei-fan,GUO Chen   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China
  • Received:2009-11-05 Revised:2010-02-06 Online:2010-06-01 Published:2010-12-29
  • Contact: YUE Xi-qing E-mail:minzhongman@gmail.com

摘要:

通过Plackett-Burman 设计法对影响屎肠球菌(Enterococcus faecium)BC-3 产类细菌素发酵培养基的10 个因素进行评价,筛选出具有显著效应的3 个因素:蛋白胨、牛肉膏、柠檬酸二铵。用最陡爬坡路径逼近类细菌素最大产量响应区域,并通过响应面分析法确定主要影响因素的最佳水平组合为蛋白胨33.1g/L、牛肉膏55.6g/L、柠檬酸二铵4.9g/L。优化后培养基发酵液抑菌圈直径达到17.13mm,比优化前提高了50.79%。

关键词: 屎肠球菌, 类细菌素, Plackett-Burman 设计, 响应面法, 优化

Abstract:

The production of bacteriocin-like substances by Enterococcus faecium BC-3 fermentation was optimized with respect to the effects of various fermentation culture medium components. Plackett-Burman (PB) design was employed to evaluate the importance of 10 factors affecting the production of bacteriocin-like substances, and peptone, beef extract and dibasic ammonium citrate were selected as the most significant affecting factors. Subsequently, path of steepest ascent was coupled with response surface analysis based on central composite design to investigate the optimal values of the selected factors. The optimal fermentation culture medium for the production of bacteriocin-like substances was composed of 33.1 g/L peptone, 55.6 g/L beef extract and 4.9 g/L dibasic ammonium citrate. A maximum diameter of inhibition zone of 17.13 mm obtained using such a medium, 50.79% higher than before the optimization.

Key words: Enterococcus faecium, bacteriocin-like substance, Plackett-Burman design, response surface methodology, optimization

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