食品科学 ›› 2010, Vol. 31 ›› Issue (11): 178-181.doi: 10.7506/spkx1002-6630-201011038

• 生物工程 • 上一篇    下一篇

鲫鱼肠道细菌菌群初步分析

侯进慧,陈宏伟,曹泽虹,高兆建,蔡 侃   

  1. 徐州工程学院食品(生物)工程学院
  • 收稿日期:2009-09-21 出版日期:2010-06-01 发布日期:2010-12-29
  • 通讯作者: 侯进慧 E-mail:houjinhui0@126.com
  • 基金资助:

    江苏省高校自然科学基础研究面上项目(07KJD550202);徐州市科技计划项目(XZZD0924);
    徐州工程学院引进人才科研启动项目;徐州工程学院科研项目(XKY2008110)

Preliminary Analysis of Bacterial Flora of the Intestinal Tract of Carp

HOU Jin-hui,CHEN Hong-wei,CAO Ze-hong,GAO Zhao-Jian,CAI Kan   

  1. College of Food Engineering (Bioengineering), Xuzhou Institute of Technology, Xuzhou 221008, China
  • Received:2009-09-21 Online:2010-06-01 Published:2010-12-29
  • Contact: HOU Jin-hui E-mail:houjinhui0@126.com

摘要:

从健康鲫鱼肠道分离出62 株细菌菌株,革兰氏染色分析结果表明,其中18 株为革兰氏阳性菌株,44 株为革兰氏阴性菌株。实验分析各菌株产纤维素酶和产淀粉酶的情况。筛选到26 株产淀粉酶菌株,占筛选菌株的41.94%,没有从鲫鱼肠道内筛选到产纤维素酶菌株。使用16S rDNA 基因序列检测,确定相关菌株分别属于Aeromonas、Shewanella、Pseudomonas 等。分析产酶活性较高的菌株F2 的致病性和产酶活力,确定其不是鲫鱼致病菌,其分泌性淀粉酶在pH7、温度32℃时表现出最大酶活力。

关键词: 鲫鱼, 肠道, 细菌, 淀粉酶, 共生菌

Abstract:

In this study, 62 bacterial strains were isolated from the intestinal tract of healthy Carp, Carassius auratus. Totally 18 Gram-positive and 44 Gram-negative strains were identified through Gram-staining. Cellulase and amylase production analysis verified 26 amylase-producing strains and no cellulase-producing strains in the intestinal tract of carp. Amylase-producing strain was 41.94% among all the isolated strains. Several strains were identified to be Aeromonas, Shewanella and Pseudomonas through 16S rDNA gene sequencing analysis. Pathogencity and amylase activity of strain F2 was analyzed to reveal that this strain was nonpathogenic for carp and its activity reached the peak level at pH 7 and 32 ℃.

Key words: Carassius auratus, intestinal tract, bacteria, amylase, symbiotic strain

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