食品科学 ›› 2010, Vol. 31 ›› Issue (9): 27-29.doi: 10.7506/spkx1002-6630-201009007

• 基础研究 • 上一篇    下一篇

微波处理对枯草杆菌芽孢致死效果研究

黄 明,黄 峰,周兴虎,邢 悦,周光宏   

  1. 南京农业大学 国家肉品质量安全控制工程技术研究中心
  • 收稿日期:2009-09-15 修回日期:2010-03-01 出版日期:2010-05-01 发布日期:2010-12-29
  • 通讯作者: 黄明 E-mail:mhuang@163.com
  • 基金资助:

    国家“863”计划项目(2008AA100804-5);江苏省青蓝工程项目(苏教师2008-30);江苏省科技支撑计划项目(SBC200960038)

Lethal Effect of Microwave Treatment on Bacillus subtilis var. niger Spores

HUANG Ming,HUANG Feng,ZHOU Xing-hu,XING Yue,ZHOU Guang-hong   

  1. (National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China)
  • Received:2009-09-15 Revised:2010-03-01 Online:2010-05-01 Published:2010-12-29
  • Contact: HUANG Ming E-mail:mhuang@163.com

摘要:

通过比较不同温度和时间下微波和水浴对枯草杆菌黑色变种芽孢的致死效果,以探讨微波杀菌的热效应与非热效应。枯草杆菌黑色变种芽孢活化后,制成104CFU/mL 的孢子悬浮液,然后分别用微波和水浴加热对芽孢进行灭菌,最后测定灭菌后的单位体积菌落总数,计算致死率。结果表明,微波和水浴加热在92℃时的致死率都高于85℃时的致死率。在相同的温度和时间下,微波的杀菌效果好于水浴加热,存在非热效应。只有在92℃,20min 时,微波和水浴加热对芽孢的致死率差异不显著,非热效应不明显。

关键词: 微波, 杀菌, 热效应, 非热效应, 枯草杆菌黑色变种芽孢

Abstract:

In order to explore the thermal effect of microwave sterilization, lethal effects of microwave and water-bath heating treatments on Bacillus subtilis var. niger spores were compared at various heating temperatures and time. After the activation of Bacillus subtilis var. niger spores, the spore suspension at the concentration of 104 CFU/mL was prepared. Microwave and waterbath heating treatments were used for the sterilization of spores to evaluate the lethal rate. Results indicated that the lethal rate at 92 ℃ was higher than that at 85 ℃ for both microwave and water-bath heating treatments. At the identical condition of heating temperature (85 ℃) and time, the lethal effect of microwave treatment was much better than that of water-bath heating treatment, which was due to an obvious non-thermal effect. However, no obvious difference in lethal rates of microwave and water-bath heating treatments at 92 ℃ for 20 min was exhibited.

Key words: microwave, sterilization, thermal effect, non-thermal effect, Bacillus subtilis var. niger spore

中图分类号: