低氧通气对发芽粟谷中γ - 氨基丁酸含量的影响

doi:10.7506/spkx1002-6630-201009012

食品科学 ›› 2010, Vol. 31 ›› Issue (9): 49-53.doi: 10.7506/spkx1002-6630-201009012

低氧通气对发芽粟谷中γ - 氨基丁酸含量的影响

白青云^1,2，曾波²，顾振新^2,*

1.淮阴工学院生命科学与化学工程学院 2.南京农业大学食品科技学院

收稿日期:2009-09-23 出版日期:2010-05-01 发布日期:2010-12-29
通讯作者: 顾振新 E-mail:guzx@njau.edu.cn
基金资助:
江苏省高新技术项目(BG2004323)

Effect of Hypoxic Ventilation onγ-Aminobutyric Acid Content in Germinated Foxtail Millet

BAI Qing-yun1,2，ZENG Bo2，GU Zhen-xin2,*

1. School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huai’an 223003, China；
2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received:2009-09-23 Online:2010-05-01 Published:2010-12-29
Contact: GU Zhen-xin2 E-mail:guzx@njau.edu.cn

摘要/Abstract

摘要：

通过分析粟谷发芽期间主要营养物质和γ- 氨基丁酸(GABA)含量变化，探讨粟谷中GABA 富集情况。采用低氧通气的方法对发芽粟谷中游离氨基酸、可溶性蛋白、谷氨酸、GABA 含量和谷氨酸脱羧酶(GAD)活性的动态变化进行研究。结果表明：低氧通气条件下，发芽粟谷芽长增长，但低于对照；游离氨基酸含量急剧增长并高于对照；可溶性蛋白含量先升高后下降但低于对照；谷氨酸含量在处理24h 内升高，随后下降；GAD 活性呈先升高后下降趋势，并显著高于对照；GABA 含量随处理时间显著增加，处理48h 时，GABA 含量达到24.32mg/100g，是未处理的4.78 倍，比同期对照提高87.51%。由此表明，低氧通气显著提高了发芽粟谷中GABA 含量，且与游离氨基酸含量和GAD 活性显著正相关。

关键词: 发芽粟谷, 低氧通气, γ- 氨基丁酸, 主要物质

Abstract:

In order to provide theoretical references for the deep development of millet, γ-Aminobutyric acid (GABA) accumulation in foxtail millet was explored through analyzing the change of major nutritional components and GABA content during the germination of millet. Dynamic changes of free amino acids, soluble proteins, glutamic acid, GABA content and glutamate decarboxylase (GAD) activity in germinated millet were investigated by the treatment of hypoxic ventilation. Results showed that sprout length of germinated millet were increased under hypoxic ventilation, however, the sprout length was still lower than that of the control; the content of free amino acids exhibited a rapid increase and higher than that of the control; the content of soluble proteins exhibited an initial increase and then a decrease, and lower than that of the control; an increase in glutamic acid content during first 24 h treatment of hypoxic ventilation and a following decrease were observed; GAD activity revealed a trend of initial increase and following reduction, and markedly higher than that of the control; GABA content revealed an increase during 48 h treatment of hypoxic ventilation and following decline. The maximal content of GABA was 24.32 mg/100g FW, which were 4.78 folds compared with pre-treatment, and higher 87.51% than the control at the same treatment time. These investigations indicated that hypoxic ventilation significantly improved GABA content of germinated millet, and GABA content exhibited a significantly positive correlation with free amino acids and GAD activity.

Key words: germinated foxtail millet, hypoxic ventilation, γ-aminobutyric acid, essential substance

中图分类号:

TS210.2

白青云1,2，曾波2，顾振新2,*. 低氧通气对发芽粟谷中γ - 氨基丁酸含量的影响[J]. 食品科学, 2010, 31(9): 49-53.

BAI Qing-yun1,2，ZENG Bo2，GU Zhen-xin2,*. Effect of Hypoxic Ventilation onγ-Aminobutyric Acid Content in Germinated Foxtail Millet[J]. FOOD SCIENCE, 2010, 31(9): 49-53.

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低氧通气对发芽粟谷中γ - 氨基丁酸含量的影响

Effect of Hypoxic Ventilation onγ-Aminobutyric Acid Content in Germinated Foxtail Millet

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