食品科学 ›› 2010, Vol. 31 ›› Issue (9): 113-116.doi: 10.7506/spkx1002-6630-201009027

• 基础研究 • 上一篇    下一篇

鲍鱼壳水溶性基质抗氧化活性研究

梁红宝1,郭 葳1,董声雄1,伍久林1,彭臻菲1,刘志彬1,张其清1 , 2 ,*   

  1. 1.福州大学生物和医药技术研究院 2.中国医学科学院生物医学工程研究所
  • 收稿日期:2009-09-11 修回日期:2010-01-04 出版日期:2010-05-01 发布日期:2010-12-29
  • 通讯作者: 张其清 E-mail:zhangqiq@126.com

Antioxidant Activity of Water-soluble Matrix from Abalone Shell

LIANG Hong-bao1,GUO Wei1,DONG Sheng-xiong1,WU Jiu-lin1,PENG Zhen-fei1,LIU Zhi-bin1,ZHANG Qi-qing1,2,*   

  1. 1. Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China ;
    2. Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Tianjin 300192, China)
  • Received:2009-09-11 Revised:2010-01-04 Online:2010-05-01 Published:2010-12-29
  • Contact: ZHANG Qi-qing1,2,* E-mail:zhangqiq@126.com

摘要:

考察鲍鱼壳水溶性基质(WSMA)抗氧化活性。利用DEAE-Sephadex A-25 离子交换层析法(IEC)对WSMA进行纯化。以珍珠水溶性基质(WSMP)及VC 为对照,通过对二苯代苦味肼基自由基(DPPH·)、羟自由基(·OH)、超氧阴离子自由基(O2·)的清除能力和Fe3+ 还原能力的研究考察其抗氧化作用。结果表明,WSMA 与WSMP 具有相似的Fe3+ 还原能力和O2·清除能力,而对DPPH·和·OH 无清除作用。通过IEC 纯化得到的AⅡ组分为主要活性成分,当质量浓度为1.0mg/mL 时,对O2·清除率达80%,与0.1mg/mL 的VC 相当。

关键词: 鲍鱼壳, 水溶性基质, 抗氧化性, 离子交换层析

Abstract:

The water-soluble matrix from abalone shell (WSMA) was purified by ion exchange chromatography (IEC) in DEAESephadex A-25 column. The antioxidant activity of WSMA was evaluated by scavenging capabilities of DPPH (1,1-diphenyl- 2-picrylhydrazy), hydroxyl (·OH) and superoxide anion (O2·) free radicals, and reducing powder of ferric ions. Compared with water-soluble matrix from pearl (WSMP) and vitamin C, WSMA revealed the similar reducing power and scavenging capability to O2· with WSMP, however, both WSMA and WSMP exhibited a weak inhibition effect on DPPH· and ·OH. The fraction from the second peak (AⅡ) separated by IEC was the major bioactive component. The scavenging capability of 1.0 mg/mL AⅡ to superoxide anion free radicals was 80%, which was equivalent to 0.1 mg/mL vitamin C.

Key words: abalone shell, water-soluble matrix, antioxidant activity, ion exchange chromatography

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