食品科学 ›› 2010, Vol. 31 ›› Issue (9): 150-155.doi: 10.7506/spkx1002-6630-201009035

• 生物工程 • 上一篇    下一篇

冬枣采后病害拮抗菌的筛选和鉴定

耿海峰1,张丽珍2,*,牛 伟3   

  1. 1.山西大学生物工程学院 2.山西大学生命科学与技术学院 3.山西省农业科学院
  • 收稿日期:2009-09-03 出版日期:2010-05-01 发布日期:2010-12-29
  • 通讯作者: 耿海峰 E-mail:ghf030483@163.com
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD01A16);山西省青年基金项目(2008021036-1);
    山西省留学人员科研项目(2008-119);太原市科技局项目(2008)

Screening and Identification of Antagonistic Bacteria against Post-harvest Diseases of Jujube Fruits

GENG Hai-feng1,ZHANG Li-zhen2,*,NIU Wei3   

  1. 1. College of Biological Engineering, Shanxi University, Taiyuan 030006, China;2. College of Life Science and Technology, Shanxi
    University, Taiyuan 030006, China;3. Shanxi Academy of Agricultural Science, Taiyuan 030006, China
  • Received:2009-09-03 Online:2010-05-01 Published:2010-12-29

摘要:

从采集的土样中分离筛选11 株对冬枣细交链格孢病菌、多隔镰孢霉和美澳核果褐腐串珠霉具有较好抑制作用的拮抗菌。通过平板对峙生长法测抑菌半径。结果表明:其中B26 的抑菌效果最显著,对细交链格孢的平均抑菌圈半径为15.8mm,抑制率达78.8%,对冬枣多隔镰孢霉和美澳核果褐腐串珠霉的平均抑菌圈半径分别为14.3mm 和12.8mm,抑制率分别达71.3% 和63.8%。通过在PDA 生长的形态学观察、生理生化实验以及PCR产物测序结果综合分析,初步鉴定B26 为枯草芽孢杆菌。

关键词: 冬枣, 病害, 拮抗菌, 筛选, 鉴定

Abstract:

Eleven strains of antagonistic bacteria were isolated from soil samples and exhibited an excellent antagonistic effect on Alternaria alternate, Fusarium decemcellulare Brick and Monilia fructicola. Results showed strain B26 had the strongest inhibition effect on three selected fungal pathogens. The average radius of inhibition zone was 15.8 mm in plate inhibition test and inhibition rate against Alternaria alternate was 78.8%. Similarly, strain B26 also exhibited inhibition effect on Fusarium decemcellulare Brick and Monilia fructicola with average radius of 14.3 mm and 12.8 mm, and inhibition rate of 71.3 % and 63.8%, respectively. Strain B26 was primarily identified as Bacillus subtilis through the characterization of morphological features, cultural characteristic observation PDA, physiological and biochemical measurements and 16S rDNA sequence analysis of PCR products.

Key words: Jujube fruit, post-harvest diseases, antagonistic bacteria, screening, identification

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