食品科学 ›› 2010, Vol. 31 ›› Issue (9): 197-201.doi: 10.7506/spkx1002-6630-201009044

• 生物工程 • 上一篇    下一篇

不同乳酸菌菌株抗氧化能力的比较研究

王曦1,罗霞2,许晓燕2,余梦瑶2,喻春莲3,江 南2,曾 瑾2,杨志荣1   

  1. 1.四川大学生命科学学院,生物资源与生态环境教育部重点实验室 2.四川省中医药科学院 3.四川省农业科学院
  • 收稿日期:2009-08-10 出版日期:2010-05-01 发布日期:2010-12-29
  • 通讯作者: 杨志荣 E-mail:bioyang@163.com
  • 基金资助:

    四川省青年科技基金项目(08ZQ026-014)

Comparative Studies on Antioxidant Activities of Different Lactic Acid Bacterial Strains

WANG Xi1,LUO Xia2,XU Xiao-yan2,YU Meng-yao2,YU Chun-lian3,JIANG Nan2,ZENG Jin2,YANG Zhi-rong1,*   

  1. 1. Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Science, Sichuan University,
    Chengdu 610065, China;2. Sichuan Province Chinese Medicine Institute, Chengdu 610041, China;3. Sichuan Academy of
    Agricultural Sciences, Chengdu 610066, China
  • Received:2009-08-10 Online:2010-05-01 Published:2010-12-29
  • Contact: YANG Zhi-rong E-mail:bioyang@163.com

摘要:

目的:比较不同乳酸菌菌株的抗氧化能力,为其天然抗氧化剂的开发提供理论依据。方法:通过羟自由基清除实验,超氧阴离子自由基清除实验,DPPH 自由基清除实验以及还原能力测定实验,对35 株乳酸菌的菌体、无细胞提取物以及胞外分泌物的抗氧化能力进行研究。结果:比较发现La 5 和La 29 两株乳酸菌抗氧化能力相对较高。结论:不同来源的乳酸菌菌株还原能力不同,且在羟自由基、超氧阴离子自由基和DPPH 自由基的清除能力方面存在差异;且其发挥抗氧化作用的活性物质存在的部位也因菌株的不同而具有较大的差异性。

关键词: 乳酸菌, 抗氧化能力, 自由基, 还原能力

Abstract:

Objective: In order to provide theoretical evidences for developing novel natural antioxidants, antioxidant activities of different lactic acid bacterial strains were compared in this paper. Methods: The antioxidant capabilities and reducing activities of 35 lactic acid bacterial strains from intact cells, cell-free extracts and extracellular secretions were evaluated through scavenging capabilities of hydroxyl, superoxide anion and DPPH free radicals. Results: La 5 and La 29 displayed the excellent antioxidant activity among 35 lactic acid bacterial strains. Conclusion: Different fractions from lactic acid bacteria have different scavenging capabilities in vitro. Therefore, lactic acid bacteria from difference resources, concentrations and excretion places also exhibited different antioxidant activities.

Key words: actic acid bacteria, antioxidant activity, free radicals, reducing activity

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