食品科学 ›› 2010, Vol. 31 ›› Issue (9): 211-215.doi: 10.7506/spkx1002-6630-201009047

• 生物工程 • 上一篇    下一篇

水菖蒲内生菌分离与抗菌活性菌株筛选

周晓坤1,陈 钧1,*,韩邦兴1,2,何 佳1,刘长风1   

  1. 1.江苏大学药学院 2.植物细胞工程安徽省工程技术研究中心
  • 收稿日期:2009-07-13 修回日期:2010-01-15 出版日期:2010-05-01 发布日期:2010-12-29
  • 通讯作者: 陈钧 E-mail:yxchenjun@126.com

Screening and Inhibitory Activity of Endophytic Fungi from Acorus calamus L.

ZHOU Xiao-kun1,CHEN Jun1,*,HAN Bang-xing1,2,HE Jia1,LIU Chang-feng1   

  1. 1.College of Pharmacy, Jiangsu University, Zhenjiang 212013, China;2. Anhui Engineering Technology Research Center of Plant
    Cell Engineering, Lu’an 237012, China
  • Received:2009-07-13 Revised:2010-01-15 Online:2010-05-01 Published:2010-12-29
  • Contact: CHEN Jun E-mail:yxchenjun@126.com

摘要:

对水菖蒲内生真菌进行分离,并进行抗菌活性的筛选。采用改良氯化三苯四氮唑显色(TTC)微量稀释法筛选抗菌活性菌株。以18 种常见导致食品腐败的细菌、酵母菌和霉菌为指示菌研究活性菌株的抗菌能力。结果显示,水菖蒲内存在广泛的具有抗菌活性的内生菌,占内生菌总数的40.7%,高活性菌株占内生菌总数的12.39%。其中S9 菌株发酵液具有抗细菌、酵母菌和丝状真菌的广谱抗菌能力,是潜在的食品防腐抗菌资源。

关键词: 水菖蒲, 内生菌, 抗菌活性

Abstract:

In order to isolate and screen endophytic fungi with inhibitory activity from Acorus calamus L., active strains were screened through modified TTC micro-dilution method. MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) were detected using 18 food spoilage strains. Results indicated that 40.7% endophytes exhibited antimicrobial activity and 12.39% endophytes exhibited higher antimicrobial activity. In addition, strain S9 exhibited the broadspectrum antimicrobial activity to bacteria, yeast and mycelia fungi. Active endophytic strains from A. calamus L. are potential resources for food preservation.

Key words: Acorus calamus L., endophytic fungi, antibacterial activity

中图分类号: