黄伞子实体多糖的初步纯化及降血脂研究

doi:10.7506/spkx1002-6630-201009060

食品科学 ›› 2010, Vol. 31 ›› Issue (9): 268-271.doi: 10.7506/spkx1002-6630-201009060

黄伞子实体多糖的初步纯化及降血脂研究

李德海¹，王志强¹，孙常雁²，王萍^{1 ,*}

1.东北林业大学林学院 2.黑龙江省乳品工业技术开发中心

收稿日期:2009-09-21 出版日期:2010-05-01 发布日期:2010-12-29
通讯作者: 王萍 E-mail:wangpingwobs@163.com

Purification and Hypolipidemic Effect of Polysaccharides from Fruit Body of Pholiota adipose

LI De-hai1，WANG Zhi-qiang1，SUN Chang-yan2，WANG Ping1,*

(1. School of Forestry, Northeast Forestry University, Harbin 150040, China ；
2. Heilongjiang Dairy Industry Technical Development Center, Harbin 150086, China)

Received:2009-09-21 Online:2010-05-01 Published:2010-12-29
Contact: WANG Ping1,* E-mail:wangpingwobs@163.com

摘要/Abstract

摘要：

目的：探讨黄伞子实体多糖的纯化方法及对高脂血症小鼠降血脂作用。方法：子实体多糖经中性蛋白酶和S e v a g 法联合除蛋白，用高脂饲料建立高脂血症模型，给药高、中、低剂量组分别为4 0 0 、2 0 0 、100mg/(kg bw·d)，5 周后测定小鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)。结果：黄伞子实体多糖能显著降低TC(高、低剂量组P ＜ 0.05)、TG(中剂量组P ＜ 0.05，低、高剂量组P ＜ 0.01)、LDL-C(中、高剂量组P ＜ 0.05，低剂量组P ＜ 0.01)水平，提高HDL-C 水平(低、高剂量组P ＜ 0.05，中剂量组P ＜ 0.01)和动脉粥样硬化因子(AI)(高剂量组P ＜ 0.05)。结论：黄伞子实体多糖具有降低小鼠高血脂症、预防动脉粥样硬化的作用。

关键词: 黄伞, 多糖, 高脂血症, 降血脂, 小鼠

Abstract:

Objective: To explore the purification method and hypolipidemic effect of polysaccharides from fruit body of Pholiota adipose polysaccharides. Methods: The crude polysaccharides were purified after removing protein by neutral protease and Sevag method. Experimental mice were divided into five groups including NS control group, the model group with high fat diet, the treatment groups fed with purified polysaccharides at the low-dosage of 100 mg/(kg bw·d), the medium-dosage of 200 mg/(kg bw·d) and the high-dosage of 400 mg/(kg bw·d). Due to the high fat diet, the model of hyperlipidemia was successfully established. After five weeks, the contents of serum TC, TG, high-density cholesterol (HDL-C), low-density cholesterol (LDL-C) were determined. Results: Purified polysaccharides from Pholiota adipose at three dosages exhibited the significant decrease in TC (high-dosage group and low-dosage group P＜0.05), TG (medium-dosage group P＜0.05, high-dosage group and low-dosage group P＜0.01), LDL-C (medium-dosage group and high-dosage group P＜0.05, low-dosage group P＜0.01), the significant improvement in HDL-C (high-dosage group and low-dosage group P＜0.05，medium-dosgage group P＜0.01) and arteriosclerosis factor (AI) (high-dosage group P＜0.05). Conclusion: Polysaccharides from Pholiota adipose play an important role in the reduction of blood lipid and prevention of arteriosclerosis in mice.

Key words: Pholiota adipose, polysaccharides, hypolipidemia, hypolipidemic effect, mice

中图分类号:

TQ929.2

李德海1，王志强1，孙常雁2，王萍1,*. 黄伞子实体多糖的初步纯化及降血脂研究[J]. 食品科学, 2010, 31(9): 268-271.

LI De-hai1，WANG Zhi-qiang1，SUN Chang-yan2，WANG Ping1,*. Purification and Hypolipidemic Effect of Polysaccharides from Fruit Body of Pholiota adipose[J]. FOOD SCIENCE, 2010, 31(9): 268-271.

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黄伞子实体多糖的初步纯化及降血脂研究

Purification and Hypolipidemic Effect of Polysaccharides from Fruit Body of Pholiota adipose

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