食品科学 ›› 2010, Vol. 31 ›› Issue (7): 105-108.doi: 10.7506/spkx1002-6300-201007023

• 基础研究 • 上一篇    下一篇

苦荞籽粒蛋白质营养评价及其对硒的响应

田秀英,李会合   

  1. 重庆文理学院生命科学与技术学院
  • 收稿日期:2009-07-08 出版日期:2010-04-01 发布日期:2010-12-29
  • 通讯作者: 田秀英 E-mail:cqflower@163.com
  • 基金资助:

    重庆市自然科学基金项目(2007BB1457);重庆市教委科学技术研究项目(KJ091205)

Nutritional Evaluation of Protein in Tartary Buckwheat Grain and Its Response to Selenium

TIAN Xiu-ying,LI Hui-he   

  1. College of Life Science and Technology, Chongqing University of Arts and Sciences, Chongqing 402168, China
  • Received:2009-07-08 Online:2010-04-01 Published:2010-12-29
  • Contact: TIAN Xiu-ying E-mail:cqflower@163.com

摘要:

采用盆栽实验研究苦荞籽粒蛋白质营养评价对硒的响应。结果表明,不施硒时,苦荞籽粒必需氨基酸与氨基酸总量比值(E/T)和必需氨基酸与非必需氨基酸比值(E/N)分别为34.4% 和52.2%,与WHO/FAO 提出的E/T 约40%,E/N 约60% 相接近,苦荞蛋白质为优质蛋白。在所测定的必需氨基酸中,含硫氨基酸(蛋氨酸+ 胱氨酸)含量和各评分值均最低,是第一限制性氨基酸。除施硒量0.5mg/kg 土壤处理外,施硒使苏氨酸和赖氨酸含量有不同程度升高,蛋氨酸+ 胱氨酸随外源硒质量分数的增加而降低;施硒量≥ 1.0mg/kg 时,苦荞籽粒的粗蛋白(CP)、必需氨基酸、非必需氨基酸和氨基酸总量随施硒量的增加而增加;施硒量1.5~2.0mg/kg 土壤时,除含硫氨基酸(蛋氨酸+ 胱氨酸)外其余各必需氨基酸的含量和各评价指标均优于不施硒的处理。

关键词: 苦荞, 硒, 蛋白质, 营养评价

Abstract:

Pot-culture experiment was conducted to nutritional evaluation of protein in tartary buckwheat grain and its response to selenium. Results indicated that the ratios of E/T (essential amino acid/total amino acid) and E/N (essential amino acid/nonessential amino acid) in buckwheat grain cultured in soil without selenite were 34.4% and 52.2%, respectively, which was similar with WHO/FAO standard (40% for E/T and 60% for E/N). Meanwhile, proteins in buckwheat grain exhibited excellent quality. The content, amino acid score (AAS) and chemical score (CS) of sulfur amino acids (methionine + cystine) exhibited the lowest level in all determined essential amino acids. This demonstrates that they were the first limited amino acid. However, an increase of threonine and lysine contents and a reduction of sulfur amino acids (Met + Cys) were observed in tartary buckwheat cultured in soil containing 0.5 mg/kg selenite. In addition, when soil supplemented with selenite larger than 1.0 mg/kg, crude protein, essential amino acids, non-essential amino acids and total amino acids in tartary buckwheat grain exhibited an increase. Compared to the control without selenite treatment, the soil supplemented with selenite in the range of 1.5-2.0 mg/kg could result in an obvious enhancement in ASS and CS of all other essential amino acids except methionine and cystine in tartary buckwheat grain.

Key words: tartary buckwheat, selenium, protein, nutritional evaluation

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