酱油酿造米曲霉AS 3.951(沪酿3.042)种曲胞外蛋白谱鉴定与分析

doi:10.7506/spkx1002-6300-201007040

食品科学 ›› 2010, Vol. 31 ›› Issue (7): 182-185.doi: 10.7506/spkx1002-6300-201007040

酱油酿造米曲霉AS 3.951(沪酿3.042)种曲胞外蛋白谱鉴定与分析

潘力，苗小康，梁燕嫦

华南理工大学生物科学与工程学院

收稿日期:2009-08-05 出版日期:2010-04-01 发布日期:2010-12-29
通讯作者: 潘力 E-mail:btlipan@scut.edu.cn
基金资助:
“十一五”国家科技支撑计划项目(2007BAK36B03)；广东省科技计划项目(2007A010900001)

Identification of Extracellular Protein Profile of Aspergillus oryzae AS 3.951 Grown on Wheat Bran Koji

PAN Li，MIAO Xiao-kang，LIANG Yan-chang

School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China

Received:2009-08-05 Online:2010-04-01 Published:2010-12-29
Contact: PAN Li E-mail:btlipan@scut.edu.cn

摘要/Abstract

摘要：

分析米曲霉AS 3.951 (沪酿3.042)种曲培养过程中不同培养时间的胞外蛋白谱，确定培养时间为48h 时种曲达到蛋白鉴定要求；采用基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF/TOF MS)技术检测鉴定出SDS-PAGE胶图上11 个蛋白条带，通过比对数据库鉴定得到8 个蛋白，其中包括α - 淀粉酶、淀粉糖化酶B、碱性蛋白酶、酸性蛋白酶等，与已在2009 年Biosci Biotechnol Biochem 期刊发表的《Analysis of extracellular proteins of Aspergillusoryzae grown on soy sauce koji》米曲霉AS 3.951 大曲的分泌蛋白谱共同提供了米曲霉沪酿AS 3.951 在酱油制曲过程中较完整的胞外分泌蛋白谱。

关键词: 米曲霉AS 3.951, 种曲, 胞外蛋白, 基质辅助激光解吸/ 串联电离飞行时间质谱

Abstract:

Aspergillus oryzae AS 3.951 is the most widely used strain in soy sauce manufacture in China, but the extracellular protein profile during the culture of wheat bran koji have not been identified. Totally 11 protein bands in SDS-PAGE were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and searching in Swissport protein database and Dogan genome database. According to the comparison with databases, 8 proteins including amylase, glucoamylase and proteases were identified. The extracellular protein profile of Aspergillus oryzae AS 3.951 during soy sauce manufacture were completed through the combination of our data and previous reports.

Key words: Aspergillus oryzae AS 3.951, wheat bran koji, extracellular protein profile, MALDI-TOF/TOF MS

中图分类号:

TS264.2

潘力，苗小康，梁燕嫦. 酱油酿造米曲霉AS 3.951(沪酿3.042)种曲胞外蛋白谱鉴定与分析[J]. 食品科学, 2010, 31(7): 182-185.

PAN Li，MIAO Xiao-kang，LIANG Yan-chang. Identification of Extracellular Protein Profile of Aspergillus oryzae AS 3.951 Grown on Wheat Bran Koji[J]. FOOD SCIENCE, 2010, 31(7): 182-185.

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酱油酿造米曲霉AS 3.951(沪酿3.042)种曲胞外蛋白谱鉴定与分析

Identification of Extracellular Protein Profile of Aspergillus oryzae AS 3.951 Grown on Wheat Bran Koji

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