食品科学 ›› 2010, Vol. 31 ›› Issue (7): 199-204.doi: 10.7506/spkx1002-6300-201007044

• 生物工程 • 上一篇    下一篇

松香基功能高分子稀土离子配合物固定化淀粉酶

雷福厚,卢建芳,赵 慷,覃冬梅,姚兴东   

  1. 广西民族大学化学与生态工程学院
  • 收稿日期:2009-02-16 修回日期:2009-12-15 出版日期:2010-04-01 发布日期:2010-12-29
  • 通讯作者: 雷福厚 E-mail:leifuhou@163.com
  • 基金资助:

    广西科学研究与技术开发基金资助项目(桂科攻0630004-1;桂科攻08395003-9)

Immobilization of Amylase on Rare Earth Coordinated Rosin Based Functional Polymers

LEI Fu-hou,LU Jian-fang,ZHAO Kang,QIN Dong-mei,YAO Xing-dong   

  1. School of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, China
  • Received:2009-02-16 Revised:2009-12-15 Online:2010-04-01 Published:2010-12-29
  • Contact: LEI Fu-hou E-mail:leifuhou@163.com

摘要:

分别以聚马来松香己二醇酯、马来松香乙二醇丙烯酯聚合物和马来松香乙二醇丙烯酯- 丙烯酸共聚物为载体,稀土离子为桥键配离子固定化淀粉酶。测定固定化淀粉酶的性能,探讨固定化酶反应机理。结果表明,稀土离子作为桥键配离子的固定化淀粉酶中,PMGAE Y(III)En 和PMGAEDy(III)En 效果较好;最适宜温度均为60℃,最适宜pH 值均为6.03;重复使用4 次后,活性分别为31.49、29.76mg/g·5min。此外,建立了固定化酶活性与功能高分子微孔面积及酸值间的数学关系式。

关键词: 松香基功能高分子, 固定化酶, 淀粉酶, 稀土离子

Abstract:

With polyhexmethylene maleic rosinate, polyethylene maleic rosinate polymer or its copolymer with acrylic acid as matrix, amylase was immobilized on La(III), Eu(III), Tb(III), Y(III) or Dy(III) coordinates of the matrix. The performance of the immobilized enzyme and the mechanism of immobilization were investigated. High activities were observed for the Y(III) and Dy(III) coordinates of the functional polymers. The optimal temperature and pH were both 60 ℃ and 6.03, respectively. The residual activities after used for 4 cycles were 31.49 and 29.76 mg/g·5 min. In addition, the correlation between the activities of the immobilized enzymes and surface area of the matrix as well as pH was established.

Key words: rosin based functional polymers, enzyme immobilization, amylase, rare earth ion

中图分类号: