食品科学 ›› 2010, Vol. 31 ›› Issue (7): 256-259.doi: 10.7506/spkx1002-6300-201007056

• 营养卫生 • 上一篇    下一篇

辅酶Q10、大豆甙元以及卵磷脂对小鼠体内抗氧化体系的影响

步文磊1,赵显峰2,荫士安2,王 茵1 ,*   

  1. 1.浙江省医学科学院 2.中国疾病预防控制中心营养与食品安全所
  • 收稿日期:2009-02-12 修回日期:2009-12-07 出版日期:2010-04-01 发布日期:2010-12-29
  • 通讯作者: 王茵 E-mail:wy3333@163.com
  • 基金资助:

    浙江省卫生高层次创新人才培养资助项目(2007 年);浙江省“151”人才工程第二层次培养资助项目(2006 年)

Effects of Coenzyme-Q10, Daidzein and Lecithin on Antioxidation System in Mice

BU Wen-lei1,ZHAO Xian-feng2,YIN Shi-an2,WANG Yin1,*   

  1. 1. Zhejiang Academy of Medical Science, Hangzhou 310013, China;
    2. Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China
  • Received:2009-02-12 Revised:2009-12-07 Online:2010-04-01 Published:2010-12-29
  • Contact: WANG Yin E-mail:wy3333@163.com

摘要:

目的:通过辅酶Q10、大豆甙元、卵磷脂单独以及三者联合补充,观察对小鼠体内抗氧化体系的影响。方法:采用灌胃方法,各组持续给予受试物6 周。取小鼠血清、肝以及脑组织进行与抗氧化相关的生化检验,大脑海马区神经细胞做膜脂流动性检测。结果:单独补充辅酶Q10、卵磷脂能显著降低小鼠血清中MDA 含量,单独补充大豆甙元能显著降低小鼠肝组织中MDA 含量(P < 0.05);联合补充能显著提高肝组织中GSH-Px 活性、脑及肝组织中T-SOD 活性以及显著降低血清、脑以及肝组织中MDA 含量(P < 0.05);单独(辅酶Q10 除外)及联合补充均能显著提高小鼠大脑神经细胞膜脂流动性(P < 0.05)。结论:辅酶Q10、大豆甙元及卵磷脂均能不同程度的改善小鼠体内抗氧化体系、脂质代谢以及大脑神经细胞膜脂流动性,且体现一定的联合协同效应。

关键词: 辅酶Q10, 大豆甙元, 卵磷脂, 抗氧化, 联合协同

Abstract:

Objectives: To evaluate the effects of coenzyme-Q10, daidzein and lecithin alone or their combination on antioxidation system in mice. Methods: After 6 weeks of consecutive administration with the materials tested by gastric perfusion method, mice were sacrificed and their blood serum, liver and brain tissues were harvested to conduct biochemical tests related to antioxidation system. Besides, membrane lipid fluidity of brain neuron in hippocampus region was measured. Results: Individual administration of coenzyme-Q10 and lecithin resulted in a significant decrease of serum MDA content in mice. Compared with control mice, a significant decrease of MDA content in liver tissue was observed in mice administered daidzein alone (P<0.05); in addition, GSH-Px activity in liver tissue, T-SOD activity in brain and liver tissues and MDA content in blood serum and brain and liver tissues revealed an obvious increase in mice with combined administration (P<0.05); membrane fluidity of brain neuron in hippocampus region exhibited a significant amelioration in mice with individual administration of daidzein and lecithin or combined administration (P < 0.05). Conclusion: Individual administration of coenzyme-Q10, daidzein and lecithin can improve antioxidation system, lipid metabolism and membrane fluidity of brain neuron in mice. A synergistic effect among them is also achieved.

Key words: coenzyme-Q10, daidzein, lecithin, antioxidation, synergistic effect

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