大球盖菇提取物对CCl4所致急性肝损伤小鼠的抗氧化酶活性及同工酶的影响

doi:10.7506/spkx1002-6300-201007058

食品科学 ›› 2010, Vol. 31 ›› Issue (7): 263-268.doi: 10.7506/spkx1002-6300-201007058

大球盖菇提取物对CCl4所致急性肝损伤小鼠的抗氧化酶活性及同工酶的影响

王峰¹，王晓炜¹，陶明煊¹，张超英²，程光宇^{2 ,*}

1.南京师范大学金陵女子学院 2.南京师范大学生命科学学院

收稿日期:2009-07-27 出版日期:2010-04-01 发布日期:2010-12-29
通讯作者: 程光宇 E-mail:mxwcgy@yahoo.com.cn
基金资助:
江苏省高校自然科学基础研究项目(07KJD550116)

Effect of Stropharia rugoso-annulata Extract (SRE) on Activities and Isozyme Profiles of Antioxidant Enzymes in Mice with Carbon Tetrachloride -induced Acute Liver Injury

WANG Feng1，WANG Xiao-wei1，TAO Ming-xuan1，ZHANG Chao-ying2，CHENG Guang-yu2,*

1. Ginling College, Nanjing Normal University, Nanjing 210097, China；
2. School of Life Science, Nanjing Normal University, Nanjing 210097, China

Received:2009-07-27 Online:2010-04-01 Published:2010-12-29
Contact: CHENG Guang-yu E-mail:mxwcgy@yahoo.com.cn

摘要/Abstract

摘要：

目的：研究大球盖菇提取物(SRE)对小鼠肝和主要脏器的抗氧化酶活性及同工酶的影响。方法：小鼠连续30d 给予低(100mg/(kg bw·d))、中(200mg/(kg bw·d))、高(400mg/(kg bw·d))剂量SRE 后，灌胃给予CCl4(80mg/(kg bw·d))建立动物急性肝损伤模型，动物处死后取肝及主要脏器测定抗氧化酶活性，采用聚丙烯酰胺梯度凝胶电泳分析CAT 和SOD 同工酶。结果：与模型组比，高剂量SRE 能显著提高肝总SOD、Mn-SOD、CuZn-SOD 及CAT、GPx 活性。主要脏器抗氧化酶活性测定表明，给予SRE 后，心和肾SOD、CAT、GPx 活性比模型组显著提高；造模后肾Mn-SOD 表现出代偿性增高，给予SRE 后能使其恢复到对照组水平。各脏器CAT 同工酶都为1 条，在造模后酶带染色密度明显降低，在给予SRE 后能恢复到正常水平。肝有1 条Mn-SOD带和3 条CuZn-SOD 带，在给予SRE 后能使这些酶蛋白含量达到或高于正常水平；心、肾有1 条Mn-SOD 带和4 条CuZn-SOD 带，SRE 能增加心、肾CuZn-SOD 酶蛋白染色密度。结论：SRE 能通过提高抗氧化酶的活性和同工酶酶蛋白含量，有效预防急性肝损伤小鼠肝脏和主要脏器的氧化损伤作用。

关键词: 大球盖菇提取物, 急性肝损伤, 抗氧化酶, 同工酶, 聚丙烯酰胺浓度梯度胶电泳

Abstract:

Objective: To evaluate the effect of Stropharia rugoso-annulata extract (SRE) on antioxidative enzyme activity and isozyme profile in liver and other major visceral organs of mice with CCl4-induced acute liver injury. Methods: After 4 days of normal feeding, CIR female mice (25 ± 2) g were used as experimental animals. Ten CIR mice orally administered distilled water were served as control group. Fifty-six more CIR mice were randomly divided into 4 groups of 14 mice each: SRE groups orally administered SRE solution in distilled water at low (100 mg/(kg bw·d)), medium (200 mg/(kg bw·d)) and high (400 mg/(kg bw·d)) doses and model group orally administered distilled water. All mice were administered for 30 consecutive days and low-, medium- and high-dose SRE groups and model group were fed water fasting diet for 16 h on the 30th day before oral administration of CCl4 dispersed in salad oil for creating CCl4-induced acute liver injury model. At 24 h postadministration, all mice were sacrificed to assay activities of antioxidant enzymes in liver, heart, kidney and spleen and analyze isozyme profile of CAT and SOD by gradient polyacrylamide gel electrophoresis. Results: Compared with the model group, high-dose SRE group exhibited significantly increased activities of total SOD, Mn-SOD, CuZn-SOD, GPx and CAT in liver; In addition, there was a significant increase of activities of SOD, CAT and GPx in heart and kidney in three SRE groups. Compared with the normal group, the model group exhibited a compensatory increase of Mn-SOD in kidney; however, Mn-SOD activity was decreased to the normal group level after SRE administration. One CAT band was detected in liver, kidney and heart and CCl4-induced acute liver injury resulted in a significant decrease of staining intensity of the brand. In contrast, the staining intensity of the band was increased to the normal group level after SRE administration. Similarly, one Mn-SOD and three CuZn-SOD bands were detected in liver and the staining intensity of the isozymes was also increased to the normal group level after SRE administration. One Mn-SOD and four CuZn-SOD bands were detected in heart and kidney and SRE administration resulted in the increase of staining intensity of CuZn-SOD bands. Conclusion: SRE can protect liver and other major visceral organs of mice with CCl4-induced acute oxidative injury by enhancing the activities of antioxidant enzymes and the contents of isozymes.

Key words: Stropharia rugoso-annulata extract, acute liver injury, antioxidant enzyme, isozyme, gradient polyacrylamide gel electrophoresis

中图分类号:

S646.19

王峰1，王晓炜1，陶明煊1，张超英2，程光宇2,*. 大球盖菇提取物对CCl4所致急性肝损伤小鼠的抗氧化酶活性及同工酶的影响[J]. 食品科学, 2010, 31(7): 263-268.

WANG Feng1，WANG Xiao-wei1，TAO Ming-xuan1，ZHANG Chao-ying2，CHENG Guang-yu2,*. Effect of Stropharia rugoso-annulata Extract (SRE) on Activities and Isozyme Profiles of Antioxidant Enzymes in Mice with Carbon Tetrachloride -induced Acute Liver Injury[J]. FOOD SCIENCE, 2010, 31(7): 263-268.

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大球盖菇提取物对CCl4所致急性肝损伤小鼠的抗氧化酶活性及同工酶的影响

Effect of Stropharia rugoso-annulata Extract (SRE) on Activities and Isozyme Profiles of Antioxidant Enzymes in Mice with Carbon Tetrachloride -induced Acute Liver Injury

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