食品科学 ›› 2010, Vol. 31 ›› Issue (6): 220-222.doi: 10.7506/spkx1002-6630-201006052

• 分析检测 • 上一篇    下一篇

新疆沙枣果实不同部位总酚的含量测定

胡君萍1,杨建华1 , 2,王新玲1,木艾塔尔·努尔麦麦提1,热娜·卡斯木1   

  1. 1.新疆医科大学药学院 2.新疆医科大学附属肿瘤医院
  • 收稿日期:2009-05-14 出版日期:2010-03-15 发布日期:2010-12-29
  • 通讯作者: 胡君萍 E-mail:hjp-yft@163.com
  • 基金资助:

    新疆医科大学科研创新基金项目(200806)

Determination of Total Polyphenols in Different Parts of Elaeagnus angustifolia Fruit Growing in Xinjiang

HU Jun-ping1,YANG Jian-hua1,2,WANG Xin-ling1,MUAITAER Nuermaimaiti1,RENA Kasimu1   

  1. 1. College of Pharmacy, Xinjiang Medical University, Urumqi 830054, China;
    2. The Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, China
  • Received:2009-05-14 Online:2010-03-15 Published:2010-12-29
  • Contact: HU Jun-ping1 E-mail:hjp-yft@163.com

摘要:

以没食子酸为对照品,采用Folin-Ciocalteu 比色法测定新疆不同产地沙枣果肉及种子中总酚含量。没食子酸在2~12μg/mL 范围内与吸光度呈良好线性关系(r = 0.9991),平均回收率为98.80%,RSD 为1.81%。7 个产地沙枣种子中的总酚含量均明显高于果肉,种子总酚含量/ 果肉总酚含量约为3~5 倍。实验方法简便、快速、准确,可用于沙枣果实的质量控制。

关键词: 沙枣果实, 总酚, Folin-Ciocalteu 比色法

Abstract:

Gallic acid was used as a standard substance to determine total polyphenol contents in different parts of Elaeagnus angustifolia fruit growing in Xinjiang Autonomous Region by Folin-Ciocalteu method. The calibration curve of gallic acid presented good linearity within the concentration range of 2-12 μg/mL (r = 0.9991). The average of three replicates of spike recovery for total polyphenols at three levels was 98.80%, with a relative standard deviation of 1.81%. Elaeagnus angustifolia seeds exhibited significantly higher total polyphenol content than Elaeagnus angustifolia flesh, with a 3- to 5-fold increase. The method demonstrates the advantages of convenience, rapidity and accuracy, thereby providing an effective approach for the quality control of Elaeagnus angustifolia fruit.

Key words: Elaeagnus angustifolia fruit, total polyphenols, Folin-Ciocalteu colorimetry

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