FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 348-354.doi: 10.7506/spkx1002-6630-201024077

• Analysis & Detection • Previous Articles     Next Articles

Quantification of All-trans-lycopene and β-Carotene from Tomato and Its Products by Internal Standard Method on C30-HPLC

DING Jing,HUI Bo-di*   

  1. College of Applied Arts and Science, Beijing Union University, Beijing 100191, China
  • Received:2010-09-13 Revised:2010-11-15 Online:2010-12-25 Published:2010-12-29
  • Contact: Bodi HUI E-mail:bodi_hui@ygi.edu.cn

Abstract:

Objective: To establish a simultaneous determination method for all-trans-lycopene and β-carotene by internal standard on C30-HPLC. Methods: Stationary phase, YMCTM Carotenoid column C30 (4.6 mm×250 mm, SOD-5μm, Waters); mobile phase A, CH3CN-MeOH (3:1, V/V); mobile phase B, MTBE; both A and B with the addition of 0.05% triethylamine; elution, 30% B in 0-10 min, a linear increase of mobile phase B from 30% to 90% in 10-20 min and 90% mobile phase in 20-30 min; flow rate, 1 mL/min; PDA wavelength range, 300-600 nm; monitoring wavelength, 470 nm and 450 nm; injection volume, 20μL; column temperature, room temperature. Results: Minimum detection limit of this method was 0.01μg/mL. In the range of 0.3-0.6μg internal standard, relative correction factor of internal standard to all-trans-lycopene andβ-carotene were 1.36 and 1.22, respectively. The relative standard deviations of reproducibility experiments were 0.50% and 0.70%, respectively. The recovery rates of this method were higher than 99.00%. Conclusion: The established method is suitable for the determination for all-trans-lycopene and β-carotene in tomato and its products.

Key words: lycopene, β-carotene, 8'-apo-8'-β-carotenal, internal standard

CLC Number: