Characterization of Microbial Community Structure in Daqu Starter Based on Biomarkers

doi:10.7506/spkx1002-6630-201111036

Abstract

Abstract: A biochemical technique for characterizing the microbial community structure in Daqu starter was established on the basis of different biomarkers. The microbial community structure was characterized by using the content of phospholipid fatty acids (PLFAs) determined by GC-MS and the biomass of predominant microbial community in Daqu starter was estimated by the content of ergosterol. A total of 18 species of PLFAs were determined quantitatively in Daqu starter. The predominant PLFAs were 16:0, 18:2ω6,9 and 18:1ω9, which reached up to 90% of total PLFAs. The results showed that predominant microbial community in Daqu starter was fungi. According to the relative ratio between ergosterol content and fungal biomass in Daqu starter, fungal biomass in five kinds of Daqu starters was in the range of (110.45±4.60)－(218.47±11.19)μg/mg dry weight.

Key words: Daqu starter, phospholipid fatty acids, microbial community structure, ergosterol, fungal biomass

CLC Number:

Q599

QIN Zhen，ZHENG Jia，PENG Yu-wen，JIN Yang，HUANG Jun，ZHOU Rong-qing. Characterization of Microbial Community Structure in Daqu Starter Based on Biomarkers[J]. FOOD SCIENCE, 2011, 32(11): 165-170.

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Characterization of Microbial Community Structure in Daqu Starter Based on Biomarkers

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