FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (9): 99-103.doi: 10.7506/spkx1002-6630-201309022

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Preparation and Fusion of Yeast Protoplasts and Screening of Fusants with High Yield of β-Carotene

WANG Sui-lou,WANG Hai-xiang,YANG Zhi-ping,LÜ Chun-hui,BU Fen   

  1. College of Pharmacy, China Pharmaceutical University, Nanjing 210009, China
  • Received:2012-06-12 Revised:2013-04-10 Online:2013-05-15 Published:2013-05-07
  • Contact: Sui-Lou WANG E-mail:cpuwsl@126.com

Abstract:

The protoplast fusion technique between R. glutinis NR-98 and Phaffia rhodozyma Ph-1 was studied. The strain
NR-98 could grow at 28 ℃, but had a lower yield of β-carotene. In contrast, the strain Ph-1 had high yield of β-carotene, but
could not grow at 28 ℃. The results of fusion experiments showed that the fusant R-5 revealed the parent strain properties
of thermotolerance and β-carotene yield. When using glucose as substrate at 28 ℃, the fusant R-5 had a high β-carotene
yield up to 9.20 mg/L, with the enhancement of 27.42% and 53.33% when compared with strain Ph-1 and strain NR-98,
respectively. The genetic quality of fusant R-5 was good as confirmed by stability experiments.

Key words: yeasts;protoplast fusion;&beta, -carotene

CLC Number: