Optimization of Fermentation Conditions for AFB1-degrading Enzyme Production by Pseudomonas stutzeri F4

doi:10.7506/spkx1002-6630-201309052

Abstract

Abstract:

Pseudomonas stutzeri F4 with high degradation activity of AFB1 was isolated and identified previously. In
this study, the fermentation conditions for AFB1-degrading enzyme (ADE) production by this strain were optimized.
The effects of inoculum age, inoculum size, initial pH, fermentation time and temperature on ADE activity were tested
by single factor tests. Based on the results of single factor tests, the fermentation conditions were then optimized
using response surface methodology. When inoculum size and initial pH were 3% and 6.5, the optimum fermentation
condition was inoculum age of 10.47 h, fermentation time of 42.52 h and fermentation temperature of 30.28 ℃. The
ADE activity in the fermented broth reached 2768.7 U under the optimized conditions, which was 28.99% higher than
before optimization.

Key words: Pseudomonas stutzeri F4, fermentation, AFB1-degrading enzymes, response surface methodology

CLC Number:

Q939.112

YANG Wen-hua，LIU Xiao-hua，LI Wen-ming，LI Hai-xing，CAO Yu-sheng*. Optimization of Fermentation Conditions for AFB1-degrading Enzyme Production by Pseudomonas stutzeri F4[J]. FOOD SCIENCE, 2013, 34(9): 256-261.

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