FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (9): 282-286.doi: 10.7506/spkx1002-6630-201309057

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Effect of Administation of CGMP and Lactoferrin by Gavage on Colonization Resistance in the Intestine of Mice

REN Xiao-dong,CHEN Qing-sen*,LI Jun-jie,ZHOU Li-li,YAN Ya-li   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce,Tianjin 300134, China
  • Received:2013-01-23 Revised:2013-04-15 Online:2013-05-15 Published:2013-05-07

Abstract: This study explored the effect of casein glycomacropeptide (CGMP) and lactoferrin (Lf) on the logarithmic ratio of Bifidobacterium to Enterobacter (B/E) in the intestine of mice. BALB/c mice were randomly divided into eight groups: control (daily diet), placebo (0.2 mL of normal saline), low-, middle-, and high-dose CGMP treatments ( the same volume as normal saline at different concentrations), and low-, middle-, and high-dose Lf treatments (the same volume as normal saline at different concentrations). The administration period was 14 d. Fresh feces were collected from all mice before administration, on the 3rd, 7th, 11th and 15th days of administation and 6 d after the end of the administration period for enumeration of Bifidobacterium and Enterobacter after culture in selective media. Both CGMP and Lf at the middel dose stimulated the growth of Bifidobacterium but exerted a growth-inhibiting effect on Enterobacter, leading to an increase in B/E ratio, which was more obviously increased by CGMP. This study has demonstrated that both CGMP and Lf can increase the intestinal B/E ratio of mice and modulate the intestinal microflora.

Key words: CGMP, lactoferrin, gavage, vintestinal B/E ratio

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