FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (9): 296-300.doi: 10.7506/spkx1002-6630-201309060

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Isolation and Purification of a Protein from Foxtail Millet Bran and Its Anti-proliferation Activities against Cancer Cells

SHAN Shu-hua1,2,WU Hai-li1,LI Zong-wei1,YUAN Lin-jie1,ZHAO Wen-bin1,LIU Qing-wu1,GUO Mao-lin1,LI Zhuo-yu1,*   

  1. 1. Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Biotechnology,
    Shanxi University, Taiyuan 030006, China;2. Department of Biology, Taiyuan Normal University, Taiyuan 030031, China
  • Received:2012-06-06 Revised:2013-03-19 Online:2013-05-15 Published:2013-05-07
  • Contact: shan shuhua E-mail:kuaile_yezi@163.com

Abstract:

FMB, a novel protein with cancer cell proliferation inhibitory activity was purified from foxtail millet bran by
using acetone precipitation, ammonium sulfate precipitation, Q-anion exchange column, SP-cation exchange column and
protein thermal denaturation. Its anti-proliferation activities against cancer cells were studied by MTT assay. The results
showed the FMB with varied concentrations (0.025, 0.05, 0.075 μg/μL and 0.1 μg/μL) could significantly inhibit the
proliferation of human colonic-cancer cell lines (DLD1) and cervical carcinoma cell line (HeLa) in dose-dependent manner.
However, under similar conditions, no significant anti-proliferation effect was observed on normal liver cell line (HL-7702).
Inverted microscope observations revealed that the DLD1 and Hela cells displayed classic morphological features of
apoptosis, e.g., obviously shrinking, floating and partial cell death, after being treated with FMB for 48 h. By contrast, the
cell line HL-7702 had no obvious changes under similar conditions.

Key words: foxtail millet bran protein (FMB), isolation and purification, cancer cells, proliferation inhibitory activity

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