FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (11): 72-76.doi: 10.7506/spkx1002-6630-201311017

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Monosaccharide Composition Analysis of Polysaccharides from Ilex kudincha C. J. Tseng Leaves

FAN Jia-long,MA Li-ping,YE Hong,ZENG Xiao-xiong,SUN Yi   

  1. 1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
    2. College of Food and Bioengineering, Henan University of Science and Technology, Henan 471003, China
  • Received:2012-06-11 Revised:2013-04-09 Online:2013-06-15 Published:2013-06-03

Abstract:

In the present study, crude polysaccharides from Ilex kudincha C.J. Tseng (IKPS) leaves were prepared
and purified by DEAE Sepharose Fast Flow anion-exchange column chromatography. As a result, four purified
fractions were obtained and named as IKPS-1, IKPS-2, IKPS-3 and IKPS-4. The monosaccharide composition of the
fractions was analyzed by two different methods: HPLC with pre-column derivatization with 1-phenyl-3-methyl-
5-pyrazolonde (PMP) and GC after derivatization to aldononitrile acetates. All these purified polysaccharides
were mainly composed of mannose, rhamnose, galactose, arabinose, glucuronic acid and fructose. Except IKPS-4,
all other fractions contained glucose. Furthermore, IKPS and IKPS-1 also contained fructose, and IKPS, IKPS-1
and IKPS-4 contained galacturonic acid. The results of HPLC analysis were generally consistent with those of
GC analysis. However, compared to GC analysis, HPLC analysis could provide more comprehensive and reliable
information about the monosaccharide composition of polysaccharides from Ilex kudincha C.J. Tseng leaves.

Key words: Ilex kudingcha C.J. Tseng, polysaccharides, high performance liquid chromatography, pre-column derivaitzaiton, gas chromatography

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