Semen Plantaginis Extract: Antioxidant Activity and Protective Effect on Nerve Cell Death

doi:10.7506/spkx1002-6630-201311032

Abstract

Abstract:

Objective: To study the antioxidant activity of Semen Plantaginis (Semen Plantaginis Asiaticae) flavonoid extract
and polysaccharide extract as well as their protective effect on 6-hydroxydopamine (6-OHDA)-induced toxicity in SHSY5Y
nerve cells. Methods: Flavonoid was extracted under sonification while polysaccharide was extracted with hot water.
Comparison of their antioxidant activity was carried out by DPPH radical scavenging, ferric reducing antioxidant power
and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+·) assays. The cell death inhibitory effect
and toxicity of the extracts were assessed by MTT assay. Results: The extraction yields of flavonoid and polysaccharide
were 1.54% and 2.76%, respectively. Their antioxidant ability increased in a concentration-dependent manner, and the
flavonoid extract showed greater antioxidant potential than the polysaccharide extract. Both extracts effectively protected
cells against 6-OHDA induced death. Meanwhile, they were proved to be nontoxic. Conclusion: The main active compounds
in Semen Plantaginis, flavonoid and polysaccharide have strong antioxidant activity and significantly protective effect
against 6-OHDA-induced nerve cell death. Therefore, Semen Plantaginis has a promising potential as a source of natural
antioxidants and functional components that protect nerve cells from death.

Key words: Semen Plantaginis, flavonoid extract, polysaccharide extract, antioxidant, SH-SY5Y cells

CLC Number:

TS201.2

XU Li，LI Yang，LIU Xue-bo. Semen Plantaginis Extract: Antioxidant Activity and Protective Effect on Nerve Cell Death[J]. FOOD SCIENCE, 2013, 34(11): 142-146.

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