Isolation and Identification of Salmonella from Pork Industry Chains in Partial Areas of Sichuan Province

doi:10.7506/spkx1002-6630-201311054

Abstract

Abstract:

To investigate the contamination and propagation status of Salmonella in pork industry chains from Sichuan
province, and to provide a basis for food safety. CHROMagar was used for isolating Salmonella from 829 samples collected
from large-scale pig farms, downstream slaughterhouses and sale markets in Sichuan during May 2010 to May 2011.These
strains were identified by duplex PCR amplification of the invA and hut genes. Finally, serotyping of these Salmonella
isolates was performed. The results showed that 112 strains of Salmonella were identified from the above samples. Of the
samples from pig farms, slaughterhouses and sale markets, 16.40%, 10.71% and 10.00% were found to be positive for
Salmonella, respectively. A total of 11 Salmonella serotypes were found, and the dominant serotypes were S. derby,
S. typhimurium, S. potsdam and S. give. These dominant serotypes tended to spread from slaugherhouses to sale markets. As
a consequence, the hygine supervision needs to be strengthened.

Key words: Salmonella, pork industry chain, isolation and identification, serotype

HOU Xiao-gang，LIU Shu-liang，HAN Xin-feng，WU Cong-ming. Isolation and Identification of Salmonella from Pork Industry Chains in Partial Areas of Sichuan Province[J]. FOOD SCIENCE, 2013, 34(11): 250-253.

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