Maternal High-fat Diet Exposure Leads to Insulin Resistance and Impacts Skeletal Muscle and Adipose Tissue Gene Expression in Offspring-rats

doi:10.7506/spkx1002-6630-201323057

Abstract

Abstract:

To explore the molecular mechanisms of insulin resistance (IR) in offspring-rats under maternal high-fat diet
exposure, we investigated whether maternal high-fat diet consumption can result in offspring malprogramming and the onset
of metabolic diseases in adulthood, and can impact skeletal muscle and adipose tissue gene expression. Sprague-Dawley
female rats were randomly divided into two groups and fed either a normal rodent chow (NC) or high-fat diet (HF). The
body weights of the rats were monitored and plasma parameters, oral glucose tolerance test (OGTT), insulin sensitivity index
(ISI) were measured. After mating with normal adult male rats, the pups of HF and NC female rats were divided into four
groups according to dams and gender: male offspring of maternal HF (MOH), female offspring of maternal HF (FOH), male
offspring of maternal NC (MON) and female offspring of maternal NC (FON). Offspring-rats were fed NC until 18 weeks
after weaning and weighed weekly. Plasma parameters, OGTT and ISI of offspring-rats were obtained and the morphology
of nuclei and mitochondria from left quadriceps cells in offspring-rats was observed. During the embryonic and fetal period,
the expression of 10 marker genes related to adipogenesis and myogenesis was detected. The results showed that maternal
high-fat diet exposure revealed obvious IR in MOH and FOH. The expression of adipogenetic and myogenetic genes was
disrupted seriously in MOH and FOH. As a consequence, maternal high-fat diet exposure leads to IR in offspring rats
through impacting the expression of adipogenetic and myogenetic genes.

Key words: maternal high-fat diet exposure, insulin resistance, adipose tissue, skeletal muscle, gene expression, rat

CLC Number:

LAI Peng，YANG Xiao，LIU Zhen-ping，TANG Yuan-mou，RAO Su，WEN Chun-juan，JIAO Shi-rong，LEI Ji，CHEN Xiang-gui*. Maternal High-fat Diet Exposure Leads to Insulin Resistance and Impacts Skeletal Muscle and Adipose Tissue Gene Expression in Offspring-rats[J]. FOOD SCIENCE, 2013, 34(23): 282-290.

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