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Optimization of Fermentation Medium for the Production of Antimicrobial Substances by Bacillus amyloliquefaciens

YANG Shengyuan, WEI Jin, ZHENG Xieru   

  1. Department of Biological Sciences, Hanshan Normal University, Chaozhou 521041, China
  • Online:2015-06-15 Published:2015-06-07

Abstract:

The modified Landy medium used for the production of antimicrobial substances by Bacillus amyloliquefaciens
K6 was optimized by using a combination of bidirectional single factor method and orthogonal array design. The results
indicated that KCl was unfavourable for the biosynthesis of antimicrobial substances, which was little affected by FeSO4
and CuSO4 but significantly affected by KH2PO4, MgSO4 and MnSO4. The optimum medium for the yield of antimicrobial
substances consisted of 5.0 g/L L-monosodium glutamate, 15.0 g/L glucose, 0.5 g/L KH2PO4, 0.2 g/L MgSO4, and 2.0 mg/L
MnSO4. Compared with the initial Landy medium, the optimized Landy medium contained much fewer constituents but
was more suitable for the biosynthesis of antimicrobial substances.

Key words: bidirectional single factor method, Landy medium, antimicrobial substance, Bacillus amyloliquefaciens

CLC Number: