Abstract:

The ultrasonic-assisted enzymatic extraction of polysaccharides from the dried fleshy stem of Cynomorium
songaricum grown in the Hexi Corridor region, Gansu province was optimized by one-factor-at-a-time and orthogonal
array design methods for improved yield of polysaccharides. The effects of the ratio of material to water, ultrasonic treatment
time, ultrasonic power, cellulase dosage, hydrolysis temperature, time, and pH on the extraction yield of polysaccharides
were investigated. The inhibitory effects of the extracted polysaccharides on the proliferation of human cervical cancer HeLa
cells in vitro were studied. The optimal extraction conditions obtained were as follows: material to water ratio, 1:10 (g/mL);
ultrasonic treatment time, 10 min; ultrasonic power, 300 W; enzyme dosage, 1.8%; hydrolysis temperature, 60 ℃; hydrolysis time,
90 min; and initial pH, 5.5. Under these conditions, the maximum yield of polysaccharides of 3.01% was obtained. MTT assay
results showed that the polysaccharide had obvious anti-tumor activity against human cervical cancer HeLa cells.

Key words: Cynomorium songaricum, polysaccharides, extraction, ultrasonic-assisted enzymatic hydrolysis, bioactivity