FOOD SCIENCE ›› 2016, Vol. 37 ›› Issue (20): 113-119.doi: 10.7506/spkx1002-6630-201620019

• Safety Detection • Previous Articles     Next Articles

Establishment of Magnetic Bead-Based Immunosensor for the Detection of Tetracycline Residues in Milk

KANG Huaibin, MA Hongyan, LI Fang, XU Baocheng, LUO Denglin, ZHANG Ruihua, ZOU Liangliang   

  1. 1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China;
    2. Henan Engineering Research Center of Food Material, Luoyang 471003, China
  • Received:2016-10-27 Revised:2016-10-27 Online:2016-10-25 Published:2016-12-01

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Abstract:

A highly sensitive and rapid immunofluorescence assay for the detection of tetracycline residues in raw milk
based on magnetic beads was developed. The limit of detection (LOD) and limit of quantification (LOQ) of this method were
0.03 and 0.20 ng/mL, respectively. The fluorescence intensity was linear with the concentration of tetracycline in the range
of 0.25 to 32 ng/mL with a coefficient of determination of 0.995 9 (R2 = 0.995 9). The recoveries of TC spiked at different
concentrations were between 82.6% and 97.0%, indicating that the method is accurate. Furthermore, precision, calculated as
relative standard deviation (RSD), was below 5.0% for intra-day and inter-day determinations, indicating good repeatability of the
method. The results of tetracycline residues in raw milk determined by this method and HPLC-UV were highly consistent.

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