Preparation and Applicability of a Simple and Fast Test Strip to Detect Listeria monocytogenes

doi:10.7506/spkx1002-6630-201620033

FOOD SCIENCE ›› 2016, Vol. 37 ›› Issue (20): 191-197.doi: 10.7506/spkx1002-6630-201620033

• Safety Detection • Previous Articles Next Articles

Preparation and Applicability of a Simple and Fast Test Strip to Detect Listeria monocytogenes

ZHENG Xiaofeng, HAN Yong, LIU Yingyu, ZHANG Juntao, ZHANG Qi, ZHANG Xiaohong, YAO Gang

1. College of Veterinary Medicine, Xinjiang Agricultural University, ürümqi 830052, China;
2. ürümqi Station of Animal Husbandry and Pasture of Xinjiang Province, ürümqi 830000, China

Received:2016-10-27 Revised:2016-10-27 Online:2016-10-25 Published:2016-12-01
Contact: YAO Gang

Abstract

Abstract:

Purpose: In the present study, a paper-based test strip for the fast and simple detection of foodborne L.
monocytogenes (LM) was prepared and named as LM-FTP (Patent Acceptance Number: 201510274726.9), and its
performance was evaluated. Methods: The performance evaluation was carried out in comparison with 3M PetrifilmTM by
using them to detect samples of meat, milk, drinking water and vegetable collected from farmer’s market and supermarket.
Results: The specificity of LM-FTP was 100% with a limit of detection of 2.1 × 104 CFU/mL, and the detection process from
bacterial enrichment to obtainment of analytical results took about 5.5 hours. Both LM-FTP and 3M PetrifilmTM could enable
the detection of the samples we tested. The coincidence rate between the results obtained using LM-FTP and 3M PetrifilmTM
for the detection of livestock products and vegetables from farmer’s market was about 75%, whereas that for those from
supermarket was about 62.5%. In addition, the coincidence rate between the results of both methods for supermarket delis
was about 93.75%. Conclusions: LM-FTP is simpler to prepare, easier to use, and time saving compared with 3M PetrifilmTM,
but it was inferior to 3M PetrifilmTM in terms of sensitivity. Therefore, LM-FTP remains to be further improved.

Key words: Listeria monocytogenes, enzyme substrate method, rapid test stripx

CLC Number:

S852.612

ZHENG Xiaofeng, HAN Yong, LIU Yingyu, ZHANG Juntao, ZHANG Qi, ZHANG Xiaohong, YAO Gang. Preparation and Applicability of a Simple and Fast Test Strip to Detect Listeria monocytogenes[J]. FOOD SCIENCE, 2016, 37(20): 191-197.

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Preparation and Applicability of a Simple and Fast Test Strip to Detect Listeria monocytogenes

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