Abstract: This paper develops an immunomagnetic bead-based enzyme-linked immunosorbent assay (ELISA) for the detection of carbaryl. Under optimum conditions, the method exhibited a good linear relationship in the range of 1 × 10-3-10 mg/L, with a correlation coefficient (R2) of 0.994. The maximum inhibition rate reached 90.6%, and the halfmaximum inhibitory concentration (IC50) was 0.077 mg/L. The detection limit (LOD) of this method was 1.48 × 10-3 mg/L. The precision for three replicate detections of carbaryl (1 mg/L) was 1.67% (relative standard deviation, RSD). The recovery of rice and Chinese cabbage spiked with carbaryl was between 70.5% and 123.1%. Also, it was found that the detection results were highly correlated with those obtained by high performance liquid chromatography (R2 = 0.91). In conclusion, the immunomagnetic bead-based ELISA can be used for rapid detection of carbaryl.

Key words: carbaryl, immunomagnetic beads, enzyme-linked immunosorbent assay (ELISA), detection, correlation