FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (14): 316-322.doi: 10.7506/spkx1002-6630-201714049

• Safety Detection • Previous Articles    

Purification Efficiency of Four Fusarium Toxins from Wheat Flour with Different Purification Columns

ZHANG Jianing, DING Ke, HAN Tao, CHEN Xiangning   

  1. (Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms and Pesticides, Beijing Laboratory of Food Quality and Safety, Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China)
  • Online:2017-07-25 Published:2017-09-06

Abstract: A method for the simultaneous determination of four Fusarium toxins in wheat flour including zenralenone, deoxynivalenol, T-2 and HT-2 toxins was established using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Samples were cleaned up with a MycoSep?226 multifunctional column, a DZT MS-PREP immunoaffinity column, which were both commercial products, and a polystyrene-divinylbenzene (PS-DVB) immunoaffinity column which was prepared in our laboratory, respectively. The recoveries of Fusarium toxins purified with MycoSep?226 ranged from 69.42% to 111.12% with relative standard deviations (RSDs) of 1.46%–13.24%, those obtained with PS-DVB ranged from 70.05% to 112.80% with RSDs of 3.42%–12.25%, and those obtained with DZT MS-PREP ranged from 88.01% to 107.31% with RSDs of 1.09%–14.42%. Immunoaffinity columns had the advantages of repeated use, less organic solvent consumption and high specificity. Moreover, PS-DVB could be a promising new immunoaffinity carrier.

Key words: Fusarium toxins, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), MycoSep?226 multifunctional column, polystyrene-divinylbenzene (PS-DVB) immunoaffinity column, DZT MS-PREP immunoaffinity column

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