Abstract: An efficient ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the simultaneous analysis of residues of 8 antiviral drugs (arbidol, amantadine, rimantadine, famciclovir, peramivir, imiquimod, oseltamivir, and lamivudine) in chicken meat and liver and pork meat and liver. Samples were extracted with 1% formic acid-acetonitrile, and the extract was subjected to chromatographic separation on MCX solid-phase extraction column. Quantification of the analytes was achieved by UPLC-MS/MS using the internal standard method. The limit of detection (LOD) and limit of quantification (LOQ) of the method were lower than 1.0 and 3.0 μg/kg for all the antiviral drugs, respectively. The average recoveries of 8 antiviral drugs in blank chicken meat, pork meat, chicken liver and pork liver at spiked levels of 5.0, 10.0, and 20.0 μg/kg ranged from 70% to 120%, with relative standard deviation (RSD) of less than 10%. The method had the advantages of small matrix interference, high sensitivity and good recovery. Therefore, it can be used for the detection of antiviral drugs residues in animal-derived food.

Key words: antiviral drugs, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), chicken meat, chicken liver, pork meat, pork liver