FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (7): 40-46.doi: 10.7506/spkx1002-6630-20180319-241

• Basic Research • Previous Articles     Next Articles

Preparation of Antioxidant Peptide from Moringa oleifera Seeds and Its Protective Effects on Oxidatively Damaged Erythrocytes

LIN Lianzhu, ZHU Qiyuan, ZHAO Mouming   

  1. School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
  • Online:2019-04-15 Published:2019-05-05

Abstract: An antioxidant peptide was purified from enzymatic hydrolysate of Moringa oleifera seed proteins by antioxidant activity-guided separation using fractional precipitation with 20%, 40% and 60% ethanol and macroporous resin column chromatography. Simulated gastrointestinal digest of the antioxidant peptide was evaluated for oxygen radical absorbance capacity (ORAC). The peptide profile and structure of the digest was identified by ultra performance liquid chromatographyquadrupole time of flight-tandem mass spectrometry (UPLC-QTOF-MS/MS). The anti-hemolysis activity and protective effect of the digest against 2,2’-azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative damage in erythrocytes were studied. The 60% ethanol precipitate was harvested and purified using column chromatography by sequential elution with pure water, 20%, 40% and 60% ethanol, yielding an antioxidant peptide rich in antioxidant and hydrophobic amino acids (which contained 92.84% protein and exhibited 1,1-diphenyl-2-picrylhydrazyl scavenging capacity and ORAC value of 39.95 and 1 454.57 μmol/g, respectively). The antioxidant activity of the peptide was enhanced after gastrointestinal digestion, and short-chain peptides including 11 dipeptides and 5 tripeptides were released. The resulting digest, Gln-Met and Leu-Phe could effectively inhibit oxidative stress-induced hemolysis through scavenging intracellular reactive oxygen species (ROS), down-regulating malondialdehyde (MDA) level and superoxide dismutase (SOD) activity, and inhibiting AAPH-induced oxidative stress.

Key words: Moringa oleifera seed, antioxidant peptides, bioaccessibility, structure identification, oxidatively damaged erythrocytes

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