FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (10): 141-148.doi: 10.7506/spkx1002-6630-20180621-405

• Bioengineering • Previous Articles     Next Articles

Screening for Broad-Spectrum Lytic Phage against Vibrio parahaemolyticus and Its Application to Marine Products

ZHENG Xiaoshuang1, GAO Lu1,2, ZHANG Hui3, RAO Shengqi1,2, YANG Zhenquan1,2,*   

  1. 1. College of Food Science and Engineering, Yangzhou University, Yangzhou 225009, China; 2. Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China; 3. Key Laboratory for Food Quality and Safety of Jiangsu Province-State Key Laboratory Cultivation Base by Province and Ministry of Science and Technology, Institute of Food Safe and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Online:2019-05-25 Published:2019-05-31

Abstract: Five lytic bacteriophages were isolated from sewage samples using pathogenic Vibrio parahaemolyticus (Vp) strains as hosts. The biological and antibacterial properties of the isolated bacteriophages were studied using artificially contaminated yellow croaker as a model. Transmission electron microscopy (TEM) revealed that the 5 phages belonged to the Myoviridae, Siphoviridae or Corticoviridae families. The lytic spectrum showed that the single phages could lyse 5–24 Vp strains, while their mixture (VppMIX) could lyse all 42 tested Vp strains. The activity of the phages remained stable at 60 ℃ and pH 4–10. The bacteriophage insensitive mutant frequency (BIMF) of the single phages was 10-3–10-5, while that of VppMIX was reduced to 10-6. The bacteriostatic test showed that number of Vp in artificially contaminated yellow croaker was significantly reduced by 1.41–4.98 (lg(CFU/g)) as compared with the control (P < 0.01) when treated by VppMIX at 25 ℃ for 12 h . This study provides a new biological bacteriostatic agent to control pathogenic Vp in marine products.

Key words: Vibrio parahaemolyticus, phage, broad-spectrum, bacteriostatic effect

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