Comparison of Free Amino Acids between Human and Bovine Milk at Different Stages of Lactation

doi:10.7506/spkx1002-6630-201808021

Abstract

Abstract: This study aimed to detect and compare the free amino acid composition in human and bovine milk at different lactation stages using isobaric tags for relative and absolute quantification (iTRAQ) combined with high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) (HPLC-MS/MS-iTRAQ). The results indicated that human milk contained more types and higher contents of free amino acids compared with bovine milk. In addition, the total content of free amino acids showed a declining trend during the whole lactation period. The total contents of free amino acids were 0.32, 0.16, 0.63 and 0.37 g/L in bovine colostrum, bovine milk, human colostrum and human milk, respectively. Among 42 free amino acids detected in this study, 35 were found in human milk and 31 in bovine milk. Moreover, the contents of 25 free amino acids in human milk were higher than in bovine milk. In particular, a significant increase in histidine, phenylalanine and threonine were observed (P < 0.05). The content of free glutamic acid was the highest in bovine milk, human colostrum and human milk, while taurine was the most abundant free amino acid in bovine colostrum. Understanding the differences in free amino acid composition between human milk and bovine milk can lay a theoretical foundation for detailed studies on amino acid functions and metabolomics in human milk. In addition, this study can provide a basis for producing infant milk powder and functional dairy products.

Key words: milk, human milk, iTRAQ, free amino acid

CLC Number:

TS252.1

WU Shangyi, WU Shang, HAN Hongjiao, KONG Fanhua, GUAN Boyuan, ZHANG Xitong, CAO Xueyan, KANG Shimo, TAO Dongbing, YUE Xiqing. Comparison of Free Amino Acids between Human and Bovine Milk at Different Stages of Lactation[J]. FOOD SCIENCE, 2018, 39(8): 129-134.

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Comparison of Free Amino Acids between Human and Bovine Milk at Different Stages of Lactation

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