FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (10): 312-317.doi: 10.7506/spkx1002-6630-201810047

• Safety Detection • Previous Articles     Next Articles

Screening and Identification of Mycotoxin-Producing Strains from Grains at Harvest Time

PEI Shichun1, LI Yan2, GAO Jianwei2, WANG Yan2, WANG Lin3, HAN Gidong4   

  1. (1. College of Food Science and Engineering, Tonghua Normal University, Tonghua 134002, China; 2. College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China; 3. Perfect Ease Biotech (Beijing) Co. Ltd., Beijing 101111, China; 4. College of Life Applied Science, Yeungnam University, Daegu 38541, Korea)
  • Online:2018-05-25 Published:2018-05-15

Abstract: This study aimed to screen out mycotoxin-producing fungal strains from wheat, corn and rice at harvest time in northeast China. ELISA, UPLC-MS/MS and PCR were adapted as analytic tools. Fungal isolation, liquid medium culture, primary screening of toxigenic strains, qualitative analysis of toxin production and gene sequence alignment of isolates were carried out. Six toxigenic strains producing zearalenone, deoxynivalenol and fumonisin were obtained and identified by DNA analysis. They were found to belong to four species, Fusarium asiaticum, F. poae, F. graminearum and F. fujikuroi. The results from this study can provide basic experimental data for the targeted prevention and control of toxic fungal contamination in foodstuffs.

Key words: mycotoxins, toxin-producing strains, isolation and identification, DNA sequencing, Fusarimum

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