FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (18): 262-266.doi: 10.7506/spkx1002-6630-201818040

• Safety Detection • Previous Articles     Next Articles

Simultaneous Determination of Dinotefuran and Its Metabolites in Vegetables by QuPPe-UPLC-MS/MS

WU Yancan1,2, JIANG Bingxin1, SHI Yanhong3,*, QI Chuanyong2, CAO Haiqun1, TANG Jun3, SHANG Luning2, SU Yiling2   

  1. (1. School of Plant Protection, Anhui Agricultural University, Hefei 230036, China;2. Hefei Testing and Inspection Center for Agricultural Products Quality, Hefei 230091, China;3. School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)
  • Online:2018-09-25 Published:2018-09-18

Abstract: A method for the simultaneous identification and quantification of dinotefuran and its metabolites 1-methyl-3-(tetrahydro-3-furylmethyl) guanidium?dihydrogen (DN) and 1-methyl-3-(tetrahydro-3-furylmethyl) urea (UF) in vegetables (cucumber, tomato, potato, and cabbage) was developed using quick polar pesticides (QuPPe) extraction procedure combined with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The analytes were separated on a UPLC BEH-C18 column, detected in the positive ion mode (ESI+) using multiple reaction monitoring (MRM), and quantified by an external standard method. The proposed method showed good linearity in the range of 1.25–500 μg/L with regression coefficients of higher than 0.991. The limits of detection (LODs) ranged between 0.04 and 0.66 μg/L, and the limits of quantification (LOQs) were between 0.13 and 2.20 μg/L. The average recoveries of the analytes from spiked samples of cucumber, tomato, potato, and cabbage (the lowest spiked concentration level was 10 μg/kg) were in the range of 72.0%–109.1% with good precisions (relative standard deviation, RSD = 1.4%–12.8%). The majority of the analytes exhibited moderate or strong matrix effects. Results suggested that the method is simple, accurate, efficient and useful for rapid screening and quantitative analysis of dinotefuran and its metabolites in vegetables.

Key words: dinotefuran, metabolites, quick polar pesticides (QuPPe) extraction, vegetables, ultra performance liquid chromatography-tandem mass spectrometry, matrix effects

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