FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (24): 300-305.doi: 10.7506/spkx1002-6630-20190107-085

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Determination of Three Catecholamines in Livestock Meat by Solid Phase Extraction Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

REN Nan, ZHAO Wentao, CHEN Chao, WU Yanchao, ZHANG Yingying, LI Yingying, GUO Wenping, FAN Wei, WANG Shouwei   

  1. (China Meat Food Research Center, Beijing 100068, China)
  • Online:2019-12-25 Published:2019-12-24

Abstract: This study aimed to establish a method for simultaneous qualitative and quantitative analysis of three catecholamines (CAs) in livestock meat by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were homogenized, extracted with perchloric acid solution, and purified on a PCX solid phase extraction cartridge. The chromatographic separation was achieved on an HILIC Plus column (4.6 mm × 100 mm, 3.5 μm) using a mobile phase consisting of acetonitrile and formic acid in water (1:99, V/V). The analytes were ionized by positive electrospray (ESI+), detected in the multiple reaction monitoring mode (MRM) and quantified by the external standard method. The results indicated that a good linearity over the concentration range of 40–1 000 ng/mL was observed for all the analytes with correlation coefficients greater than 0.99. The limits of detection (LOD) and quantification (LOQ) were 10 and 40 μg/kg, respectively. The average recoveries of the CAs ranged from 75.48% to 91.25% with relative standard deviations (RSDs) of 1.2%–4.6% (n = 6). The method is sensitive, simple, rapid and accurate and can meet the requirements for practical analysis.

Key words: catecholamines, livestock meat, β adrenergic agonist, solid phase extraction, ultra-high performance liquid chromatography-tandem mass spectrometry

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