Abstract: Purpose: To establish a rapid method for simultaneous determination of 29 phenols in wine by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods: The sample was diluted with acidified methanol-water, and centrifuged at high speed. The supernatant was taken for analysis. The chromatographic separation was implemented on an ACQUITY UPLC? BEH C18 column using a mobile phase consisting of 0.1% formic acid-acetonitrile and 0.1% formic acid water. The analytes were detected using an electrospray ion source with multiple reaction monitoring in the negative ion mode. The instrumental parameters were as follows: ion source temperature (TEM) 500 ℃, electrospray voltage ?4 500 V, air curtain gas (CUR) pressure, 40 psi, collision gas medium, atomized gas pressure 60 psi, auxiliary heating pressure, 50 psi. The analysis process took 17 min. Results: The limits of quantitation (LOQs) of 29 monomeric phenols were between 0.001 and 0.01 mg/L, and the linear correlation coefficients R2 were greater than 0.99. The recoveries for spiked samples ranged between 90.8% and 104.5%, with relative standard deviations (RSDs) of 0.26% to 7.54%. Conclusion: This method is characterized by simple pretreatment, high sensitivity and fast analysis as well as good precision and accuracy, and thuscan be applied to detect 29 monophenols in wine.

Key words: mono-phenolic compounds, wine, ultra-high performance liquid chromatography-tandem mass spectrometry