FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (5): 173-179.doi: 10.7506/spkx1002-6630-20190311-135

• Nutrition & Hygiene • Previous Articles     Next Articles

Anti-fatigue Effect and Molecular Mechanism of Black Ginseng Polysaccharides in Mice

LIU Xinglong, ZHAO Yingchun, CHEN Xueyan, SHE Xinxin, SADIA Khatoon, JIANG Ying, XIE Huiyong, JIANG Bainan, ZHENG Yinan, LIU Wencong, DING Chuanbo   

  1. (1. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; 2. Jilin Jianwei Natural Biotechnology Co. Ltd., Linjiang 134300, China; 3. Shenzhidao (Tonghua) Biotechnology Co. Ltd., Tonghua 134001, China)
  • Online:2020-03-15 Published:2020-03-23

Abstract: Objective: To investigate the effect of black ginseng polysaccharides (BGP) on alleviating physical fatigue in mice and to explain the underlying mechanism. Methods: A mouse model of muscle fatigue was established by weight-bearing swimming and the mice were orally administered simultaneously with BGP at 50, 100 or 200 mg/(kg mb·d) for 28 days. Exhaustive swimming time was recorded. Then, the levels of blood lactic acid (BLA), blood urea nitrogen (BUN) and serum interleukin-6 (IL-6) as well as the contents of glycogen and malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver were measured. The expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) was evaluated by using Western blot; the expression of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) and atherosclerosis peroxisome proliferator activated receptory α (PPARα) in skeletal muscle was analyzed by reverse transcription quantitative real-time polymerase chain reaction. Results: Compared with the weight-bearing swimming control group, BGP significantly prolonged exhaustive swimming time (P < 0.05), and it significantly decreased the levels of BLA, BUN and IL-6 and MDA content in liver (P < 0.05), improved the activities of SOD and GSH-Px and the content of glycogen (P < 0.05), inhibited the expression of IL-1β and TNF-α, and increased the expression levels of PGC-1α and PPARα compared with the non-weight-bearing swimming control group (P < 0.05). Conclusion: BGP have good anti-fatigue effect by regulating the expression of PGC-1α and PPARα genes in skeletal muscle and inhibiting inflammatory reaction in liver.

Key words: black ginseng polysaccharides, anti-fatigue effect, oxidative stress, inflammatory factors, gene expression

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