Isolation, Purification and Identification of Dipeptidyl Peptidase-4 Inhibitor from Lactic Acid Bacterial Metabolite

doi:10.7506/spkx1002-6630-20190411-138

Abstract

Abstract: In this study, the metabolites of Lactobacillus plantarum BLP12 with inhibitory activity against dipeptidyl peptidase-4 (DPP-4) were separated and purified by organic solvent extraction, ultrafiltration membrane, gel chromatography and high performance liquid chromatography. The substrate N-glycyl-prolyl-p-nitroanilide hydrochloride (Gly-Pro-pNA) was applied to determine DPP-4 activity based on the strong absorption at around 405 nm of the degradation product of Gly-Pro-pNA. The fraction with strong inhibitory activity was selected for chemical composition analysis by liquid chromatography tandem mass spectrometry. A total of 61 substances were identified including dodecyl sulfate, which has been reported as a DPP-4 inhibitor.

Key words: lactic acid bacteria, metabolite, dipeptidyl peptidase-4, inhibition activity

CLC Number:

TS252.1

DONG Jie, LIU Lu, JIA Chengli, ZHU Junling, Lü Jiaping. Isolation, Purification and Identification of Dipeptidyl Peptidase-4 Inhibitor from Lactic Acid Bacterial Metabolite[J]. FOOD SCIENCE, 2020, 41(8): 116-122.

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Isolation, Purification and Identification of Dipeptidyl Peptidase-4 Inhibitor from Lactic Acid Bacterial Metabolite

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