Development of an Electrochemical Immunosensor for Rapid Quantification of Florfenicol in Animal-Derived Foods

doi:10.7506/spkx1002-6630-20190612-127

Abstract

Abstract: An electrochemical immunosensor based on graphene-chitosan composites was established to detect florfenicol residues in animal-derived foods. A linear relationship between the peak current and the concentration of florfenicol was observed in the range of 1–1 000 ng/mL and the detection limit (RSN = 3) was 0.08 ng/mL. The immunosensor displayed good selectivity, specificity and stability. The average recoveries from pork, beef, chicken and egg samples spiked at concentration levels of 10, 100 and 1 000 μg/kg ranged from 76.90% to 94.30%. The reliability of the electrochemical method was confirmed by a high correlation between it and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The established immunosensor method was accurate and reliable and could be applied to the on-site detection of massive samples.

Key words: veterinary drug residues; florfenicol; electrochemical immunosensor; graphene-chitosan composite

CLC Number:

S859.84

LIU Weihua, YANG Xi, LIU Minxuan, YU Wenlong, WANG Xianghong. Development of an Electrochemical Immunosensor for Rapid Quantification of Florfenicol in Animal-Derived Foods[J]. FOOD SCIENCE, 2020, 41(20): 307-313.

[1]	MA Ning, WANG Jie, PEI Fei, FANG Yong, YANG Wenjian, HU Qiuhui. Determination and Risk Assessment of Veterinary Drugs in Pork Meat and Viscera during Slaughter, Pre-cooling and Sale [J]. FOOD SCIENCE, 0, (): 314-319.
[2]	Fei PEI Yong Fang Wenjian Yang qiuhui hu. Determination and risk assessment of veterinary drugs in pork during slaughtering and pre-cooling and commercial stages [J]. , 0, (): 0-0.
[3]	Fei PEI Yong Fang Wenjian Yang Qiu-Hui HU. Systematic study of the analysis of quinolones with different sample preparations in porcine tissues [J]. FOOD SCIENCE, 0, (): 0-0.
[4]	WANG Jie, PEI Fei, LI Peng, BAI Yujie, FANG Yong, YANG Wenjian, MA Ning, HU Qiuhui. Comparison of Different Sample Pretreatments for the Analysis of Quinolone Residues in Porcine Tissues [J]. FOOD SCIENCE, 2018, 39(18): 309-314.
[5]	Ting Mao. Suggestions on the Key Research Areas of Technical Support System for Food Safety Supervision during the 13th Five-Year Plan [J]. FOOD SCIENCE, 0, (): 0-0.
[6]	ZHU Liping, WANG Jianping, DING Xuemei, ZENG Qiufeng, BAI Shiping, XUAN Yue, SU Zhuowei, ZHANG Keying. Analysis of 5-Vinyl-1,3-oxazolidine-2-thione Residue in Eggs by HPLC [J]. FOOD SCIENCE, 2016, 37(18): 159-163.
[7]	. Analysis of 5-vinyl-1,3-oxazolidine-2-thione in eggs by HPLC [J]. FOOD SCIENCE, 0, (): 0-0.
[8]	MAO Ting, LU Yong, JIANG Jie, ZHANG Weimin, FENG Nan. Recent Advances in Rapid Screening and Confirmation Methods for the Risk of Unknown Chemical Substances in Foods [J]. FOOD SCIENCE, 2016, 37(5): 245-253.
[9]	ZHANG Yuan1,2, LI Weiqing1, ZHOU Weie1, REN Zhiqin, ZHANG Feng, FENG Xuesong, ZHOU Yu, LI Shaohui, ZHENG Yang. Progress in Sample Pretreatment and Analytical Techniques for the Determination of Sulfonamide Residues in Foods [J]. FOOD SCIENCE, 2015, 36(23): 340-346.
[10]	HUA Xiaoxia, ZHENG Xiangli, LIU Shan, XIA Fangquan, ZHOU Changli. Electrochemical Behavior and Determination of Bisphenol A at Carbon Paste Electrode Modified with Ionic Liquid [J]. FOOD SCIENCE, 2015, 36(22): 152-155.
[11]	. Synthesis of Artificial Antigen and Preparation of Polyclonal Antiserum Against Metronidazole [J]. FOOD SCIENCE, 0, (): 0-0.
[12]	LIU Ru-biao, HU Xiao-fei, ZHANG Gai-ping, WANG Fang-yu, DENG Rui-guang, LIU Lu, JIA Guo-chao, LIU Xi. Synthesis of Artificial Antigen and Preparation of Polyclonal Antiserum against Metronidazole [J]. FOOD SCIENCE, 2014, 35(19): 185-188.
[13]	. Simultaneous Determination of residues of Four Dipyrone Metabolites in Goat Muscle by Hydrophilic Interaction Liquid Chromatography/Mass Spectrometry [J]. FOOD SCIENCE, 0, (): 0-0.
[14]	ZHANG Chong1,2, XUE Fei-qun2, ZHANG Li-fang2, ZHANG Xiao-xiao2, WANG Xiao-yang2, JIANG Shan-xiang1,*. Simultaneous Determination of Residues of Four Dipyrone Metabolites in Goat Muscle by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry [J]. FOOD SCIENCE, 2014, 35(10): 158-162.
[15]	. etermination of Chloramphenicol and Florfenicol in Fishery Products by Using Dispersive Solid Phase Extraction and Gas Chromatography [J]. FOOD SCIENCE, 0, (): 0-0.

Development of an Electrochemical Immunosensor for Rapid Quantification of Florfenicol in Animal-Derived Foods

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments