FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (13): 133-140.doi: 10.7506/spkx1002-6630-20190617-176

• Nutrition & Hygiene • Previous Articles     Next Articles

Effects of Blueberry Anthocyanins on Lipopolysaccharide-Induced Inflammation in RAW 264.7 Cells and HCT-116 Cells Proliferation and Apoptosis

LIN Yang, YANG Ping, ZHANG Qi, LANG Yuxi, DENG Haotian, MENG Xianjun   

  1. (1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China; 2. College of Grain, Shenyang Normal University, Shenyang 110034, China)
  • Online:2020-07-15 Published:2020-07-29

Abstract: Purpose: The objective of this study was to investigate the anthocyanin composition, antioxidant, anti-inflammatory and anticancer activities of blueberry extract (BE) and to unravel the possible underlying mechanism. Methods: The anthocyanin composition was analyzed by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The levels of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interlukin-6 (IL-6), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) were determined to explore the anti-inflammatory effect. The 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromide and Western blot analyses were used to determine the inhibitory effect on cell proliferation and apoptosis of BE at different concentrations. Results: Fourteen anthocyanins were identified in BE with strong antioxidant activity. BE remarkably reduced lipopolysaccharide (LPS)-induced production of inflammatory mediators such as NO, inflammatory cytokines such as IL-6 and TNF-α, and the expression of COX-2 and iNOS. BE could significantly inhibit the proliferation of HCT-116 cells in a dose- and time-dependent manner. Furthermore, BE treatment could result in a decrease in cyclin/cyclin-dependent kinase-2 (CDK2) and an increase in p53. Conclusion: BE has the potential to inhibit inflammation and colon cancer.

Key words: inflammation bowel diseases, blueberry extracts, anthocyanin composition, antioxidant activity, inflammation, colon cancer

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