FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (15): 282-287.doi: 10.7506/spkx1002-6630-20190625-312

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Preparation of Carvacrol-Loaded Casein Nanoparticles and Its Inhibitory Activity against Colletotrichum acutatum on Loquat Fruit (Eriobotrya japonica)

HUA Chunyang, LI Zhuoye, JIN Peng, QIN Dingkui, DU Qizhen   

  1. (School of Agriculture and Food Science, Zhejiang Agricultural and Forestry University, Hangzhou 311300, China)
  • Online:2020-08-15 Published:2020-08-19

Abstract: Objective: To prepare casein (CS) nanoparticles (NPs) loaded with carvacrol (CL) for improved antifungal activity of CL. Methods: Carvacrol-loaded casein nanoparticles (CL-CS-NPs) were prepared through ultrasonic treatment. The difference in the CL retention rate of CL-CS-NPs, when incubated on solid culture medium, versus free CL and a physical mixture of casein and CL was detected by high performance liquid chromatography (HPLC). The in vitro growth inhibitory effects of free CL, casein + CL and CL-CS-NPs on Colletotrichum acutatum mycelium were evaluated as well as the inhibitory effects on Colletotrichum acutatum inoculated into loquat fruit (Eriobotrya japonica (Thunb.) Lindl. cv. Baisha). Results: Stable CL-CS-NPs (152.6 nm in diameter) could be prepared by adding CL (12 mg/mL) to casein solution (20 mg/mL) followed by ultrasonic treatment. The sustained release time of CL-CS-NPs was 4 days and 2 days longer than that of free CL group and casein + CL mixture group, respectively, indicating that nano-CL had an obvious sustained release effect, and CL-CS-NPs showed potential to prolong the time of action of CL on pathogens. The in vitro culture experiment showed that CL-CS-NPs completely inhibited Colletotrichum acutatum for 5 days, while casein + CL mixture and free CL were effective for 3 days. Loquats sprayed with CL-CS-NPs dispersion did not rot basically at 5 days after inoculation, and the average lesion diameter and disease incidence were only 1.5 mm and 10% at 7 days. Moreover, those treated with casein + CL mixture had relatively small lesions but a high disease incidence (80%) at 5 days, and seriously rotted with an average lesion diameter of up to 4.8 mm at 7 days. Conclusion: Spraying the CL-CS-NPs dispersion onto loquat fruit can have a significant inhibitory effect on anthracnose rot caused by Colletotrichum acutatum.

Key words: carvacrol, nanoparticles, loquat, anthracnose, inhibition effect

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