Abstract: For a better understanding of its metabolic mechanism and function during mutton fermentation, the whole genome of Lactobacillus helveticus TR13, a strain with high lipase activity isolated from naturally air-dried mutton was sequenced using the PacBio Illumina HiSeq sequencing platform. The results showed that the genome of TR13 was a set of circular plasmid-free molecules, and the total length of the genome sequence was 2 172 224 bp with a GC content of 36.82%. It was predicted to contain 2 536 protein-coding genes with a total length of 1 883 532 bp, an average length of 799.46 bp, and an average density of 1.08 per kb, and the average sequence length of the coded proteins was 265 amino acids (aa), accounting for 86.71% of the genome. The genome of TR13 contained 15 rRNA operons and 63 tRNA, 75 virulence genes and 150 drug resistance genes being predicted from the genome. Moreover, functional annotation of the genome and metabolic pathway information annotation were accomplished by aligning the coding genes to the GO, COG and KEGG databases, which would provide a theoretical basis to explore the application of strain TR13.

Key words: Lactobacillus helveticus; whole genome sequencing; gene function annotation