Inhibition Effect of Lignosus rhinocerotis Polysaccharides-Selenium Nanoparticles Prepared by Ultrasound Treatment on Non-enzymatic Glycosylation

doi:10.7506/spkx1002-6630-20190725-337

Abstract

Abstract: In order to explore the inhibitory effect of Lignosus rhinocerotis polysaccharides-selenium nanoparticles (LRP-SeNPs) on the non-enzymatic glycosylation reaction, stable LRP-SeNPs were synthesized under ultrasound irradiation, and the effects of LRP-SeNPs on the characteristic products (fructosamine, dicarbonyl compounds and fluorescence end products) of non-enzymatic glycosylation reaction at three stages were studied by ultraviolet spectroscopy, fluorescence spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that ultrasound treated LRP-SeNPs (U-LRP-SeNPs) could effectively inhibit non-enzymatic glycosylation reaction in a dose-dependent manner. The percentage inhibition of non-enzymatic glycosylation by U-LRP-SeNPs with Se/LRP ratio of 1:10 and 1:15 (m/m) remained at 30% and 20%, respectively. The inhibitory effect of U-LRP-SeNPs was further verified by SDS-PAGE. Ultrasound could effectively improve the stability of LRP-SeNPs, reduce the size of SeNPs, and increase the specific surface area, thereby providing more sites for binding with free radicals to shield or terminate free radicals, effectively inhibit oxidation reaction caused by free radicals, and finally prevent non-enzymatic glycation reaction at the oxidation stage.

Key words: Lignosus rhinocerotis polysaccharides-selenium nanoparticles (LRP-SeNPs), ultrasound, non-enzymatic glycosylation, inhibition effect

CLC Number:

TS254.1

XIAO Yidong, CAI Wenfei, ZHENG Zhaomin, MA Huiyu, HUANG Qilin. Inhibition Effect of Lignosus rhinocerotis Polysaccharides-Selenium Nanoparticles Prepared by Ultrasound Treatment on Non-enzymatic Glycosylation[J]. FOOD SCIENCE, 2020, 41(15): 134-139.

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Inhibition Effect of Lignosus rhinocerotis Polysaccharides-Selenium Nanoparticles Prepared by Ultrasound Treatment on Non-enzymatic Glycosylation

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