FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (14): 286-293.doi: 10.7506/spkx1002-6630-20190802-033

• Processing Technology • Previous Articles     Next Articles

Extraction of Polysaccharides from Dendrobium officinale and Their Inhibition Effect on Hydroxyl Radical-Induced Apoptosis of SH-SY5Y Cells

ZHANG Yadan, ZHAO Mengqian, YANG Yujiao, XU Youzhi, WANG Zijun, WANG Yanjun, XU Diya, ZHANG Lin, ZHOU Binbin   

  1. (1. Hunan Key Laboratory of Processed Food for Special Medical Purpose, National Engineering Laboratory of Rice and By-products Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China; 2. Hunan Institute of Food Quality Supervision Inspection and Research, Changsha 410117, China)
  • Published:2020-07-29

Abstract: The ultrasonic-assisted enzymatic extraction of polysaccharides from Dendrobium officinale was optimized using one-factor-at-a-time and orthogonal array design methods. Solid-to-liquid ratio, ultrasonication time, and enzyme concentration were considered as independent variables. High performance liquid chromatography (HPLC) was used for analyzing the monosaccharide composition of D. officinale polysaccharides. Coumarin-3-carboxylic acid (CCA) fluorescence method was employed for determining the antioxidant activity of D. officinale polysaccharides. Methyl thiazolyl tetrazolium (MTT) and flow cytometry were employed for investigating the inhibition effect of the polysaccharides on hydroxyl radical-induced apoptosis of SH-SY5Y cells. The results showed that the optimum extraction conditions were as follows: solid-to-solvent ratio 1:100 (g/mL), ultrasonication time 2 h, and enzyme (a 1:1 mixture of cellulase and pectinase, m/m) concentration 50%, and the yield of polysaccharides was 25.69% under these conditions. The monosaccharide composition analysis showed that D. officinale polysaccharides were mainly composed of mannose and glucose. D. officinale polysaccharides at concentrations ranging from 20 to 80 μg/mL could effectively scavenge hydroxyl radicals produced from hydrogen peroxide with Cu2+ as a catalyst and consequently decreased hydroxyl radical-induced apoptosis of SH-SY5Y cells (the apoptotic rate decreased from 56.97% to 17.97%) in a concentration-dependent manner.

Key words: Dendrobium officinale polysaccharide; extraction; hydroxyl radical; SH-SY5Y cells

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