Preparation of Magnetic Three-Dimensional Reduced Graphene and Its Application in Electrochemical Immunoassay of Mycobacterium tuberculosis H37Ra in Milk

doi:10.7506/spkx1002-6630-20200508-078

FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (22): 308-314.doi: 10.7506/spkx1002-6630-20200508-078

• Safety Detection • Previous Articles Next Articles

Preparation of Magnetic Three-Dimensional Reduced Graphene and Its Application in Electrochemical Immunoassay of Mycobacterium tuberculosis H37Ra in Milk

LIU Xiya, TIAN Chunmei, ZHENG Lufei, ZHONG Pingsheng, ZHANG Xinfang, REN Jiali

(1. Hunan Key Laboratory of Forestry Edible Sources Safety and Processing, Changsha 410004, China; 2. College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China; 3. Institute of Quality and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Online:2020-11-25 Published:2020-11-26

Abstract

Abstract: In this paper, an electrochemical immunosensor based on magnetic three-dimensional ferroferric oxide/reduced graphene nanocomposite (Fe3O4@3D-RGO) was developed and applied to rapidly detect Mycobacterium tuberculosis H37Ra in milk. Fe3O4@3D-RGO was synthesized by a one-step hydrothermal reduction method and then characterized by scanning electron microscopy, electron energy spectroscopy and Raman spectroscopy. A modified electrode was prepared with 75% ethanol and 0.1% Nafion solution as dispersion system. The electrochemical performance was investigated using electrochemical impedance spectroscopy (EIS). Under the optimal conditions, M. tuberculosis H37Ra was detected. The results showed that the sensor displayed a strong linear relationship between the electrode impedance and the logarithmic concentration of M. tuberculosis H37Ra in the range of 1 × 103–1 × 108 CFU/mL. The regression equation was y = 19.77lgN ? 49.14, the limit of detection (LOD) was 1 × 102 CFU/mL and the detection time was as short as 15 min. This method had strong specificity and good repeatability and stability. The one-step hydrothermal reduction method, thanks to its simplicity and environmental friendliness, provides technical support for the development of portable electrochemical immunosensors.

Key words: magnetic three-dimensional reduced graphene; modified electrode; Mycobacterium tuberculosis H37Ra; electrochemical immunosensor; rapid detection

CLC Number:

TS207.4

LIU Xiya, TIAN Chunmei, ZHENG Lufei, ZHONG Pingsheng, ZHANG Xinfang, REN Jiali. Preparation of Magnetic Three-Dimensional Reduced Graphene and Its Application in Electrochemical Immunoassay of Mycobacterium tuberculosis H37Ra in Milk[J]. FOOD SCIENCE, 2020, 41(22): 308-314.

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Preparation of Magnetic Three-Dimensional Reduced Graphene and Its Application in Electrochemical Immunoassay of Mycobacterium tuberculosis H37Ra in Milk

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