FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (8): 534-536.

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Study on Determination of Reducing Sugar Content Using 3,5-Dinitrosalicylic Acid Method

 ZHAO  Kai, XU  Peng-Ju, GU  Guang-Ye   

  1. Province Key Laboratory of Food Science and Engineering,College of Food Engineering,Harbin University of Commerce,Harbin 150076,China
  • Online:2008-08-15 Published:2011-08-26

Abstract: Two kinds of 3,5-dinitrosalicylic acid(DNS) agents were respectively used for determination of reducing sugar content,and effects on determination results by the factors,such as amount of DNS reagent developing time,wavelength and storage time were discussed. The results showed that on the conditions of reagent amount around 1.5 ml,boiling bath time not less than 5 minutes,and standing for 30 minutes after developing,at the wavelength of 540 nm and 550 nm for reagent one and two respectively,the accurate results can be acquired.

Key words: 3,5-dinitrosalicylic acid(DNS), reducing sugar, spectrophotometry, determination factor